LOCUS       sequence-369780-        3426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A30_STOP
FEATURES             Location/Qualifiers
     source          1..3426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(983..1082)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1099..1118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1100..1118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1123..1139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1123..1139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1241..1260)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1252..2061
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1319..1338)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2208..2796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2697..2716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2950..2967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3126..3153)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3245..3331)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3380..3402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3394..3411
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3395..3411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgtc cgccctgaac
      121 tggaagcctt tcgtgtacgg aggactggca tctatcaccg cagagtgcgg cacatttcca
      181 atcgacctga ccaagacaag actgcagatc cagggccaga ccaatgatgc caagttcaag
      241 gagatccggt acagaggaat gctgcacgca ctggtgcgga tcggaagaga ggagggcctg
      301 aaggccctgt attccggaat cgcaccagca atgctgaggc aggcctctta cggcaccatc
      361 aagatcggca catatcagag cctgaagaga ctgttcatcg agaggcccga ggacgagacc
      421 ctgcctatca acgtgatctg tggcatcctg agcggcgtga tcagctccac catcgccaac
      481 cccacagatg tgctgaagat ccgcatgcag gcccagtcca atacaatcca gggcggcatg
      541 atcggcaact ttatgaatat ctaccagcag gagggaacca ggggactgtg gaagggcgtg
      601 tccctgacag cacagagggc agccatcgtg gtgggcgtgg agctgcccgt gtacgacatc
      661 accaagaagc acctgatcct gtctggcctg atgggcgata ccgtgtatac acacttcctg
      721 tctagcttta cctgcggact ggcaggcgcc ctggcatcta accctgtgga cgtggtgagg
      781 acacgcatga tgaatcagag ggtgctgagg gacggccggt gcagcggcta caccggcaca
      841 ctggattgtc tgctgcagac ctggaagaat gagggcttct ttgccctgta taagggcttt
      901 tggccaaact ggctgaggct gggaccatgg aatatcatct tctttgtgac atatgagcag
      961 ctgaagaagc tggatctgta agacccagct ttcttgtaca aagttggcat tataagaaag
     1021 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1081 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1141 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1201 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1261 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1321 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1381 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1441 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1501 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1561 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1621 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1681 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1741 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1801 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1861 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1921 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1981 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2041 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2101 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3061 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3121 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3181 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3241 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3301 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3361 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3421 gggccc
//
