LOCUS       sequence-369792-        2911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Markerless yeast integration Spacer at Int.9 locus to used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT093
FEATURES             Location/Qualifiers
     source          1..2911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(824..1412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(904..923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1626..1645)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1637..2452
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1637..2452
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1710..1729)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 agctaggtaa catattcatt cgagatgtcc acctgcaccg agtagtcctc tttaaatctt
       61 ggaagaaaac acgtcagcga tgccaagatt ctacacattt ccttcgggta ctcatgtctt
      121 cgctagaaga cattgtacgt actgcagacg tactagtccg cgttactctg tttatatcgc
      181 tctagagcta gaattctttg ttggacaagg gaatcggagt gggcttcaaa ccaaaaccaa
      241 aatttcaacg tataggccat tgtgctcgat gatgaaggga ctgcaaaaat atcggtggtt
      301 taagataagc aattgtaaga tatagaagaa aagctgacga aaaaagaact cataccttac
      361 tccaaaatgg acactgacat attctcccat tcggtggatt tgaaatcctc agtggataga
      421 taggcagagt tgagccaatt tgcctaggga gttgattacc gttaatcctg cttgaaagca
      481 gcatattaaa ccttccccga agtttgccat aggaccgtat gacgcatcct ggacttcatc
      541 ccatcactgt gctgggactt gaaaataaat gtttcgcccg tggtaaaaag tgtgtgggaa
      601 taaggtagcc ctctacttct tagcatactc aaaaagagat gctaatagtc aatctacggc
      661 ctgattcagg actgcctcac ggaaaattac atcgcttcgg gattcgtgat catctggcgg
      721 ccgcacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      961 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1021 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1081 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1141 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1201 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1261 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1321 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1381 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1441 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact gatccttcaa
     1501 ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta
     1561 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1621 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcca ggccgcgatt
     1681 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1741 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1801 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1861 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1921 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga
     1981 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2041 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg cgcaatcacg
     2101 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2161 tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac
     2221 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2281 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2341 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2401 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2461 ggttaattgg ttgtaacacg cggccgccaa atccgagggt aactgtgata gcttcaaaag
     2521 acttttagca tttgaaaccg ctcagtggta tcattactcc atcaaccacc aatgcagtca
     2581 catgtttatg agaacagtat caagcacctt aattccgagg ctgtaataaa tgggacattt
     2641 tggacagaat gtctcattat ccccgaaccg aagcctttga aattgatctg caaatgaggt
     2701 aattcagctg gatggttttt cataatatat tgaacatctg ggaagaattt cacgtcacct
     2761 ctcggtttat ctcagtagta tttagagtat tcttcaagtt ctgatgggaa ttttctcgcc
     2821 atgcctgaat ttcagttaat acgtgtttaa gctccgaaat ccatgtatca aaattcactg
     2881 caaaaaggtt gtaatttgat ggaggagatc a
//
