LOCUS       sequence-369795-        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A34_STOP
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1022..1121)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1138..1157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1139..1157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1162..1178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1162..1178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1280..1299)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1291..2100
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1358..1377)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2247..2835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2736..2755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2989..3006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3165..3192)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3284..3370)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3419..3441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3433..3450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3434..3450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gaccgtgcca
      121 cctgcagtgg acctggtgct gggagcatct gcctgctgtc tggcatgcgt gttcaccaac
      181 cccctggagg tggtgaagac acggctgcag ctgcagggag agctgcaggc aaggggcaca
      241 tacccaagac cctatcacgg ctttatcgca agcgtggcag cagtggcaag ggcagatgga
      301 ctgtggggac tgcagaaggg actggcagca ggactgctgt accagggcct gatgaatggc
      361 gtgcgcttct actgctatag cctggcctgt caggcaggac tgacccagca gccaggagga
      421 acagtggtgg caggagcagt ggcaggcgcc ctgggagcct ttgtgggctc ccccgcctat
      481 ctgatcaaga cccagctgca ggcacagaca gtggcagcag tggcagtggg ccaccagcac
      541 aaccaccaga ccgtgctggg cgccctggag acaatctgga ggcagcaggg cctgctgggc
      601 ctgtggcagg gagtgggagg agcagtgcct cgcgtgatgg tgggatctgc cgcacagctg
      661 gccaccttcg ccagcgccaa ggcatgggtg cagaagcagc agtggctgcc agaggactcc
      721 tggctggtgg ccctggcagg aggaatgatc agctccatcg ccgtggtggt ggtcatgacc
      781 ccctttgacg tggtgtctac acggctgtac aatcagcctg tggataccgc aggaagagga
      841 cagctgtatg gaggactgac agactgtatg gtgaagatct ggaggcagga gggacctctg
      901 gccctgtaca agggactggg acctgcatat ctgagactgg gcccacacac catcctgtcc
      961 atgctgttct gggatgagct gaggaagctg gccggccgcg cccagcacaa gggcacataa
     1021 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1081 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1141 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1201 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1261 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1321 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1381 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1441 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1501 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1561 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1621 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1681 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1741 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     1801 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     1861 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     1921 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1981 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2041 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2101 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2161 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3121 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3181 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3241 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3301 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3361 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3421 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
