LOCUS       sequence-369799-        3576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A43_STOP
FEATURES             Location/Qualifiers
     source          1..3576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1133..1232)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1249..1268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1250..1268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1273..1289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1273..1289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1391..1410)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1402..2211
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1469..1488)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2358..2946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2847..2866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3100..3117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3276..3303)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3395..3481)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3530..3552
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3544..3561
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3545..3561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cacatggcgg
      121 agagacggca ggctgaccgg aggacagcgc ctgctgtgcg caggactggc cggcacactg
      181 tctctgagcc tgaccgcacc tctggagctg gcaacagtgc tggcacaagt gggagtggtg
      241 cggggacacg ccagaggccc atgggccacc ggccacaggg tgtggcgcgc cgagggactg
      301 agggccctgt ggaagggaaa cgcagtggca tgcctgaggc tgttcccttg ttccgccgtg
      361 cagctggccg cctacaggaa gttcgtggtg ctgtttacag acgatctggg ccacatctct
      421 cagtggagct ccatcatggc aggatctctg gcaggaatgg tgagcaccat cgtgacatac
      481 ccaaccgacc tgatcaagac ccggctgatc atgcagaata tcctggagcc cagctataga
      541 ggcctgctgc acgccttctc cacaatctac cagcaggagg gctttctggc cctgtatagg
      601 ggcgtgtctc tgaccgtggt gggcgccctg ccattttccg ccggatctct gctggtgtac
      661 atgaacctgg agaagatctg gaatggccct agagatcagt tctctctgcc acagaacttt
      721 gcaaacgtgt gcctggcagc agcagtgacc cagacactga gcttcccctt tgagacagtg
      781 aagaggaaga tgcaggccca gtccccatat ctgccacact ctggaggagt ggacgtgcac
      841 ttctccggag cagtggattg ttttcgccag atcgtgaagg cacagggcgt gctgggactg
      901 tggaacggac tgacagccaa tctgctgaag atcgtgccct acttcggcat catgtttagc
      961 accttcgagt tttgcaagag aatctgtctg taccagaacg gctatatcct gagccccctg
     1021 tcctataagc tgacccctgg agtggatcag tccctgcagc cacaggagct gagggagctg
     1081 aagaagttct ttaagacaag aaagcccaag cctaagaagc ctaccctgta agacccagct
     1141 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1201 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1261 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1321 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1381 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1441 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1501 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1561 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1621 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1681 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1741 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1801 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1861 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1921 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1981 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2041 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2101 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2161 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2221 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2281 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3241 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3301 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3361 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3421 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3481 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3541 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
