LOCUS       sequence-369800-        8418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor to install a dual surface marker - fluorescence reporter into 
            the AAVS1 safe harbor using HDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pJT055
            AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site...
FEATURES             Location/Qualifiers
     source          1..8418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     387..404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(557..562,563..580,581..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     600..622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     619..635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     619..635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        656..674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    709..1512
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1519..1544
                     /label=SA
                     /note="splice acceptor site"
     CDS             1568..1621
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1631..2029
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(2061..2078)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2067..2291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2364..2382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2400..2418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2436..2454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2472..2490
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2508..2526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2544..2562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2580..2598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2616..2634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2652..2670
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          2915..3853
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3810..3830
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3886..3904
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(3914..3925)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      3935..3944
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3941..4000
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             4772..4801
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4805..4951
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4967..5020
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5027..5036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5033..5035,5036..5038,5039..5749)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5750..5770
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5781..5902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5818..5837)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5872..5891
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5968..6804
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(7079..7870,7871..7939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7702..7721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8063..8418,1..233)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcagaatta
      661 accctcacta aagggactag tcctgcaggt ttaaacgaat tcgccctttg ctttctctga
      721 ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac
      781 ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc
      841 cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc
      901 ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct
      961 ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt
     1021 ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat
     1081 gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac
     1141 aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg
     1201 cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc
     1261 tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat
     1321 cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac
     1381 ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta
     1441 tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc
     1501 ccacagtggg gcaagcttct gacctcttct cttcctccca cagggcctcg agagatctgg
     1561 cagcggagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     1621 taggctcgag atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     1681 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     1741 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     1801 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     1861 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     1921 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     1981 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaat ctagagggcc
     2041 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     2101 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     2161 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     2221 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     2281 gggctctatg gcttctgagg cggaaagaac cagctggggg ctagcaaagc ctaggaacag
     2341 ctgcatatag cactgcagtt tactccctat cagtgataga gagtcaaaag gccatgccct
     2401 ccctatcagt gatagagaga gcagactcaa ggtggtccct atcagtgata gagagattga
     2461 gcaagagggc ctccctatca gtgatagaga atagcactgc agtttactcc ctatcagtga
     2521 tagagagtca aaaggccatg ccctccctat cagtgataga gagagcagac tcaaggtggt
     2581 ccctatcagt gatagagaga ttgagcaaga gggcctccct atcagtgata gagattgatc
     2641 cagtcagggc ctccctatca gtgatagaga agctatgtgc aggtagggct gtacagtggg
     2701 aatgcatgag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2761 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2821 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2881 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2941 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     3001 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3061 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3121 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3181 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3241 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3301 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3361 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3421 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3481 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3541 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3601 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3661 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3721 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3781 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3841 tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta accctcacta
     3901 aagggaacaa aagctggagc tcccaccgcg gtgggccacc atggagacag acacactcct
     3961 gctatgggta ctgctgctct gggttccagg ttccactggt gacgctagcg agacgggcgg
     4021 atcaggcggt cgtctcggat ccgacaaaac tcacacatgc ccaccgtgcc cagcacctga
     4081 actcctgggg ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat
     4141 ctcccggacc cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt
     4201 caagttcaac tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga
     4261 ggagcagtac aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg
     4321 gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga
     4381 gaaaaccatc tccaaagcca aagggcagcc ccgagaacca caggtctaca ccctgccccc
     4441 atcccgggag gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta
     4501 tcccagcgac atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac
     4561 cacgcctccc gtgctggact ccgacggctc cttcttcctc tacagcaagc tcaccgtgga
     4621 caagagcagg tggcagcagg ggaacgtctt ctcatgctcc gtgatgcacg aggctctgca
     4681 caaccactac acgcagaaga gcctctccct gtctccgggt aaaggaggag gtggaagcgg
     4741 aggaggagga agcggaggag gaggtagcgg agaacaaaaa ctcatctcag aagaggatct
     4801 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa
     4861 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat
     4921 cctcatcatg ctttggcaga agaagccacg ttgtacaggc agtggagagg gcagaggaag
     4981 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca ccggtcgcca ccatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc caaaaaagaa
     5761 gagaaaggta tagttaacta aacttgttta ttgcagctta taatggttac aaataaagca
     5821 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5881 ccaaactcat caatgtatct tatcatgtct gcatatgtaa attgtaagcg tctcggcctc
     5941 tagagtcgag atgacattga ttattgaact agggacagga ttggtgacag aaaagcccca
     6001 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag
     6061 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga
     6121 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg
     6181 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta
     6241 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg
     6301 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat
     6361 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt
     6421 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg
     6481 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta
     6541 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt
     6601 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc
     6661 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag
     6721 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc
     6781 tacaggggtt cctggctctg ctcttcgcct tcttgacgag ttcttctgaa ttattaacgc
     6841 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6901 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6961 attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag atccttttaa
     7021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7621 tgactggtga atactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7981 attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8401 agcccagctt ggagcgaa
//
