LOCUS       sequence-369801-        3900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-MFSD11_STOP
FEATURES             Location/Qualifiers
     source          1..3900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1457..1556)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1573..1592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1574..1592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1597..1613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1597..1613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1715..1734)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1726..2535
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1793..1812)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2682..3270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3171..3190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3424..3441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3600..3627)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3719..3805)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3854..3876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3868..3885
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3869..3885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag cccagagtcc
      121 aagaagctgt tcaacatcat catcctgggc gtggccttca tgtttatgtt caccgccttt
      181 cagacatgcg gcaacgtggc ccagaccgtg atccggtccc tgaatagaac agacttccac
      241 ggctccggct acacctctat ggccatcatc tatggcgtgt tttctgccag caatctgatc
      301 acccctagcg tggtggcaat cgtgggacca cagctgagca tgttcgcctc cggcctgttt
      361 tactccatgt atatcgccgt gtttatccag cccttccctt ggagctttta caccgcatcc
      421 gtgttcatcg gaatcgcagc agccgtgctg tggaccgcac agggcaactg tctgacaatc
      481 aattctgatg agcacagcat cggccggaac agcggcatct tctgggccct gctgcagagc
      541 tccctgttct ttggcaatct gtacatctat tttgcctggc agggcaagac acagatctcc
      601 gagtctgacc ggagaaccgt gttcatcgcc ctgacagtga tctccctggt gggcaccgtg
      661 ctgttctttc tgatccggaa gcccgattct gagaacgtgc tgggcgagga cgagtctagc
      721 gacgatcagg atatggaagt gaatgagtct gcccagaaca atctgacaaa ggccgtggac
      781 gccttcaaga agtcttttaa gctgtgcgtg accaaggaga tgctgctgct gagcatcacc
      841 acagcctaca ccggcctgga gctgacattc tttagcggcg tgtatggcac ctgtatcggc
      901 gccacaaaca agttcggcgc cgaggagaag tctctgatcg gcctgagcgg catctttatc
      961 ggaatcggag agatcctggg aggatccctg ttcggactgc tgtctaagaa caataggttt
     1021 ggaaggaacc cagtggtgct gctgggaatc ctggtgcact ttatcgcctt ctacctgatc
     1081 ttcctgaata tgcctggcga cgcaccaatc gcaccagtga agggaacaga ttcctctgcc
     1141 tatatcaaga gctccaagga ggtggccatc ctgtgcagct tcctgctggg cctgggcgac
     1201 tcctgtttta acacccagct gctgtctatc ctgggcttcc tgtactctga ggatagcgcc
     1261 cctgcctttg ccatctttaa gttcgtgcag agcatctgcg ccgccgtggc cttcttttac
     1321 tccaattatc tgctgctgca ctggcagctg ctggtcatgg tcatctttgg cttctttggc
     1381 accatctcct tctttacagt ggagtgggag gcagcagcct tcgtggcaag gggctccgat
     1441 tatcgctcta tctaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1501 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1561 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1621 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1681 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1741 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1801 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1861 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1921 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1981 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2041 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2101 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2161 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2221 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2281 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2341 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2401 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2461 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2521 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2581 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2641 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2701 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2761 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2821 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2881 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2941 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3001 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3061 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3301 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3481 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3541 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3601 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     3661 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     3721 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     3781 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     3841 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
