LOCUS       sequence-369803-        4131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-FLVCR2_STOP
FEATURES             Location/Qualifiers
     source          1..4131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1688..1787)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1804..1823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1805..1823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1828..1844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1828..1844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1946..1965)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1957..2766
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2024..2043)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2913..3501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3402..3421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3655..3672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3831..3858)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3950..4036)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4085..4107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4099..4116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4100..4116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gaacgagggc
      121 cctaatcagg aggagtctga cgatacccct gtgccagaga gcgccctgca ggcagaccca
      181 tctgtgagcg tgcacccttc cgtgtctgtg cacccaagcg tgtccatcaa cccctctgtg
      241 agcgtccatc ctagctcctc tgcccaccca tccgccctgg cacagccatc tggactggca
      301 caccctagct cctctggacc agaggatctg tctgtgatca aggtgagccg gagaaggtgg
      361 gccgtggtgc tggtgttcag ctgctactcc atgtgcaatt cctttcagtg gatccagtat
      421 ggctctatca acaatatctt catgcacttt tacggcgtga gcgccttcgc catcgactgg
      481 ctgtccatgt gctacatgct gacatatatc ccactgctgc tgcctgtggc atggctgctg
      541 gagaagtttg gcctgcgcac catcgccctg acaggatctg ccctgaactg cctgggagca
      601 tgggtgaagc tgggatccct gaagccacac ctgttccccg tgaccgtggt gggccagctg
      661 atctgtagcg tggcccaggt gttcatcctg ggcatgccat ccagaatcgc cagcgtgtgg
      721 tttggcgcca acgaggtgag cacagcctgt tccgtggccg tgttcggcaa tcagctgggc
      781 atcgccatcg gctttctggt gccccctgtg ctggtgccca atatcgagga cagggatgag
      841 ctggcctacc acatcagcat catgttctat atcatcggag gagtggccac cctgctgctg
      901 atcctggtca tcatcgtgtt taaggagaag cccaagtatc caccatccag ggcacagtcc
      961 ctgtcttacg cactgacatc tcctgatgcc agctatctgg gctccatcgc ccggctgttc
     1021 aagaacctga attttgtgct gctggtcatc acctacggcc tgaacgccgg cgccttttat
     1081 gccctgtcca cactgctgaa tagaatggtc atctggcact atccaggaga ggaggtgaac
     1141 gcaggaagga tcggcctgac catcgtgatc gcaggaatgc tgggagccgt gatcagcggc
     1201 atctggctgg accggtccaa gacctacaag gagaccacac tggtggtgta tatcatgaca
     1261 ctggtgggca tggtggtgta caccttcaca ctgaacctgg gccacctgtg ggtggtgttt
     1321 atcaccgccg gcacaatggg cttctttatg accggctatc tgcctctggg cttcgagttt
     1381 gccgtggagc tgacataccc agagtctgag ggcatcagct ccggcctgct gaatatcagc
     1441 gcccaggtgt tcggcatcat ctttaccatc tcccagggcc agatcatcga caactacggc
     1501 acaaagcctg gcaatatctt cctgtgcgtg tttctgaccc tgggagccgc cctgacagcc
     1561 ttcatcaagg ccgatctgcg ccggcagaag gccaacaagg agaccctgga gaataagctg
     1621 caggaggagg aggaggagtc taataccagc aaggtgccaa cagccgtgag cgaggatcac
     1681 ctgtaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1741 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1801 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1861 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1921 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1981 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2041 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2101 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2161 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2221 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2281 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2341 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2401 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2461 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2521 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2581 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2641 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2701 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2761 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2821 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2881 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2941 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3001 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3061 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3601 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3661 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3721 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3781 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3841 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3901 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3961 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4021 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4081 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
