LOCUS       sequence-369809-        2373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mNeonGreen as a Type 3 part to be used in the Dueber YTK and
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT020
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(740..774)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(845..1504)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1419..1438
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1686..2274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2175..2194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatggtgtc taaaggtgaa gaggataaca tggcttcttt gccagctact
       61 cacgaattgc atattttcgg ttccatcaac ggtgttgatt tcgatatggt tggtcaaggt
      121 actggtaatc caaacgacgg ttacgaagag ttgaacctga aatctacaaa aggtgacttg
      181 caattctccc catggatttt ggttccacat attggttacg gtttccacca atatttgcca
      241 tatccagatg gtatgtctcc atttcaagct gctatggttg atggttctgg ttaccaagtt
      301 catagaacca tgcaatttga agatggtgct tctttgaccg tcaactacag atatacttac
      361 gaaggttccc atattaaggg tgaagctcaa gtaaaaggta caggttttcc agctgatggt
      421 ccagttatga ctaattcttt gactgctgct gattggtgca gatccaaaaa aacttaccca
      481 aacgataaga ccatcatctc tactttcaag tggtcttaca ctactggtaa cggtaagaga
      541 tatagaagta ctgctagaac tacttacacc ttcgctaaac ctatggctgc taactacttg
      601 aagaatcaac ctatgtacgt gttcagaaag accgaattga aacactccaa aaccgagttg
      661 aacttcaaag aatggcaaaa ggctttcacc gatgttatgg gtatggatga gttgtacaag
      721 ggatcctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      781 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
      841 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      901 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      961 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1021 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1081 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1141 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1201 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1261 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1321 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1381 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1441 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1501 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1561 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     1621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1801 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2341 cctgcgttat cccctgattc tgtggataac cgt
//
