LOCUS       sequence-369829-        9224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RARA - Retinoic acid receptor gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE15
FEATURES             Location/Qualifiers
     source          1..9224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2056..2087
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2114..2123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2120..3769
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3773..3892
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3984..4572
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4037..4057)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4455..4466)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4587..4903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4754..4889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4954..4956,4957..4959,4960..5673)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4999..5020)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5260..5279)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5607..5628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5745..5978
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6050..6171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6087..6106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6141..6160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6211..6346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6366..6385)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6367..6385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6395..6412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6395..6411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6404..6426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6553..7008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6640..6659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6850..6871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7034..7138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7139..7207,7208..7999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7357..7376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8170..8758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8659..8678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8912..8929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9046..9067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9082..9087,9088..9105,9106..9112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9120..9136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9125..9147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9144..9160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9144..9160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9179..9199
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9181..9199
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcaggtc accaggaggt caaggtcacc aggaggtcaa
     1981 ggtcaccagg aggtcaaggt caccaggagg tcagatccga gaacagatct ggcctcggcg
     2041 gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt ggcaatccgg
     2101 tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
     2161 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
     2221 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
     2281 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
     2341 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
     2401 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
     2461 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
     2521 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
     2581 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
     2641 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
     2701 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
     2761 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
     2821 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
     2881 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
     2941 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
     3001 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
     3061 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     3121 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
     3181 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
     3241 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
     3301 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
     3361 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     3421 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
     3481 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
     3541 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
     3601 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
     3661 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
     3721 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtga attctcacgg
     3781 cttccctccc gaggtggagg agcaggccgc cggcaccctg cccatgagct gcgcccagga
     3841 gagcggcatg gatagacacc ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg
     3901 cgactctaga acgcgtcact gcggcttgcc tccatagcca ttcgactaca gacatcagcg
     3961 cgtaccggtt agtaatgatc gacaatcaac ctctggatta caaaatttgt gaaagattga
     4021 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4081 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4141 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4201 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4261 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4321 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4381 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4441 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4501 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4561 ccgcctcccc gcctggcgat ggtaccggtg tggaaagtcc ccaggctccc cagcaggcag
     4621 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4861 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4921 tatatccatt ttcggatctg atcagcacct agcatggtga gcaagggcga ggagctgttc
     4981 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5041 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5101 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac ctacggcgtg
     5161 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5221 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5281 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5401 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
     5461 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5521 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5581 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5641 atcactctcg gcatggacga gctgtacaag taaggtacct ttaagaccaa tgacttacaa
     5701 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5761 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5881 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5941 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6001 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6061 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6181 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6241 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6301 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6361 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6421 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6481 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6541 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6961 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     7021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8881 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8941 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9001 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9061 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9121 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     9181 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gctt
//
