LOCUS       sequence-369835-        5300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage P1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_P1
FEATURES             Location/Qualifiers
     source          1..5300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..145
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(301..1179)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1206..1490
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1413..1432
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3254..3281
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3300..3391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3392..3460,3461..4252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3610..3629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4423..5011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4912..4931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5165..5182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(5197..5300,1..37)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5263..5282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
       61 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
      121 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca
      181 gatcaattcg cgcgcgaagg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc
      241 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
      301 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
      361 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      421 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      481 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      541 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      601 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      661 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      721 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      781 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      841 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      901 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      961 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
     1021 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
     1081 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
     1141 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
     1201 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
     1261 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1321 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1381 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1441 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1501 tgggctaaca ggaggaatta accatggcag acaataaaat cacgctatcc tcggtcagga
     1561 aggcgctggc gggggttttt aaagacaacg gagaacggga caacatcctc ctgtccgcgc
     1621 tggctgtgca cggcggaagt gggtatttgt tttctcgcgc aggggcaccg gtacaactgt
     1681 ccggcttctt aggcggcaaa ccgggcgata gtggcatggc tggcgatggg ctggtggacg
     1741 gaagtcgctt tatctttgat gaagttcaac tgccagaaga tcgcttgcaa cgctatccgc
     1801 tactcgaaga gatggcggtt tacagcacga tcgccaccgc gctgaacatc catattacgc
     1861 acgcgctctc tttcgataac aagaccggac aaaccttctc tatcgtgccg gtacataacg
     1921 gaaacgatag tgactatgac gccgcgcagg cgttgtgtgg cgagctgatg aacgacatcg
     1981 ggcgaaccat caacaaagag gtcgccggat gggcatttat catgtctgta tttggggtgg
     2041 cttatgtcag gccatacgcc aaagaaggca tagggatcac gtcttttgag tgctcctatt
     2101 acacccttcc gagcttcatc aaagagttcg aggtcagcgg caacctggcg ggatttagcg
     2161 gcgattatct gaaggacgcg tcagggaaaa tggttttcgc cgatccgtgg gccattatcc
     2221 ctatgaaaat cccctactgg cggcctaagt caaaccttat gcctgtgcac actggccata
     2281 aagcatacag cttgctggat aatccggaag agcgcacgcc gattgaaacc cagaattacg
     2341 ggaccagctt gctcgaatac gcttacgagc cgtacatgaa tctgcgttca gcgatccgct
     2401 cgctgaaagc aacgcgtttt aatgcgtcga aaattgaccg aatcatcggc ctggcgatga
     2461 atagtctgga tccggttaaa gcagccgatt attcgcgcac cattactcag acgcttaaac
     2521 gagcagctga cctgatggaa agacgcgcac gtggcgcgaa taacatgcct acggtaacca
     2581 ataccctgct gcctattatg ggcgacggca agggacagat gactattgat actcagacca
     2641 tccaggctga catcaacggc attgaagaca ttctcaccta tatgcgccag ctggcggcag
     2701 cacttggcct cgattacacc ctcctggggt gggcagatca aatgtccggc gggcttggtg
     2761 aaggtggatt cctgcgcacg gcaattcagg ccgccatgcg tgcctcatgg atccagcagg
     2821 gcgtagaaga gttcattcag cgggctatcg atattcatct tgctttcaag tacggtaagg
     2881 tatacccgga aggtgatcgc ccgtacaaaa tcgaattcca ctccgttaat accgctctgc
     2941 aacaagagca caacgacaac cgcgactcgc aggcgaacta cgccaccatc gttacgcaaa
     3001 tcctcgatgc cgtcagcaat aacagcgtac tcgccaattc cgatgcattc aaacgttacc
     3061 tgttcagcga tgtgctggag attgacgaaa aaatctctga agcactggtg aacgaactga
     3121 aagcgaaaag cgaggacgac gatcacctga tggattccat catcaaaaca ccgccacagg
     3181 aactggcgca aatccttgaa tcggtcttta aagagggaaa cgataatgac taatgccagg
     3241 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     3301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3601 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     3661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3841 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3901 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3961 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4021 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4081 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4141 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4201 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4261 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4561 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5101 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5161 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     5221 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     5281 cgcatagtta agccagtata
//
