LOCUS       sequence-369836-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage N4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_N4
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      152..740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     641..660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     894..911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    926..1066
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(992..1011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1152..1174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1330..2208)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2235..2519
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2442..2461
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      4865..4892
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4911..5002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5003..5071,5072..5863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5221..5240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
       61 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
      301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      901 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
      961 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1021 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1201 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat
     1261 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     1321 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     1381 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     1441 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     1501 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     1561 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     1621 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     1681 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     1741 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     1801 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     1861 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     1921 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     1981 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     2041 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     2101 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     2161 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     2221 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     2281 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     2341 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
     2401 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     2461 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     2521 cccgtttttt gggctaacag gaggaattaa ccatggagca aaacaccgat agcatggtgc
     2581 cgctgccgga cccgagccag gcgaccaagc tgaccagctg gaaaaacgaa ctgagcctgc
     2641 aagcgctgaa ggcggacctg gatgcggcga aaccgagcca caccgcgatg atgattaagg
     2701 tgaaagagtg gaacgacctg atgcgtatcg aaggcaaggc gaaaccgccg aaggttaaag
     2761 gccgtagcca ggtgcaaccg aagctggttc gtcgtcaagc ggagtggcgt tacagcgcgc
     2821 tgaccgaacc gttcctgggt agcaacaagc tgtttaaagt gaccccggtt acctgggagg
     2881 atgtgcaagg cgcgcgtcag aacgaactgg ttctgaacta ccagttccgt accaaactga
     2941 accgtgtgag ctttatcgac aactatgttc gtagcgtggt tgacgatggt accggcattg
     3001 tgcgtgttgg ttggaaccgt gagatccgta aggagaaaca agaagtgccg gttttcagcc
     3061 tgtttccgat tcagacccaa gaacaggcgg atgcgctgca gcaagcgctg cagctgcgta
     3121 ccgataaccc gcgtggctac gaggaaaacg tggacgaggc gatcaaggaa agcgttcgtt
     3181 tctttgacga gaccggtcaa gcgacctatg cggtgcagac cggtaccacc accaccgagg
     3241 tggaagttcc gctggcgaac cacccgaccg ttgagatgct gaacccggaa aacatcatta
     3301 tcgacccgag ctgccaaggt gatattaaca aggcgatgtt cgcgatcgtg agctttgaga
     3361 cctgcaaagc ggacctgctg aaggaaaaag atcgttacca caacctgaac aagatcgatt
     3421 ggcaaagcag cgcgccggtt aacgagccgg accatgcgac caccaccccg caagaattcc
     3481 agattagcga tccgatgcgt aaacgtgtgg ttgcgtacga gtattggggt ttttgggaca
     3541 tcgagggtaa cggcgtgctg gaaccgatcg ttgcgacctg gattggtagc accctgatcc
     3601 gtctggaaaa gaacccgtat ccggatggca aactgccgtt cgtgctgatt ccgtacatgc
     3661 cggttaagcg tgacatgtat ggcgagccgg atgcggaact gctgggcgac aaccaagcgg
     3721 tgctgggtgc ggttatgcgt ggcatgatcg acctgctggg tcgtagcgcg aacggtcagc
     3781 gtggcatgcc gaaaggcatg ctggatgcgc tgaacagccg tcgttaccgt gagggtgaag
     3841 actacgagta taacccgacc caaaacccgg cgcagatgat tatcgagcac aagttcccgg
     3901 aactgccgca gagcgcgctg accatggcga ccctgcaaaa ccaggaagcg gaaagcctga
     3961 ccggcgtgaa agcgtttgcg ggtggcgtta ccggtgaaag ctatggcgat gttgcggcgg
     4021 gtattcgtgg cgttctggat gcggcgagca agcgtgagat ggcgattctg cgtcgtctgg
     4081 cgaagggtat gagcgaaatc ggcaacaaaa ttatcgcgat gaacgcggtg ttcctggcgg
     4141 agcacgaagt ggttcgtatt accaacgagg aattcgttac catcaagcgt gaggacctga
     4201 agggtaactt tgacctggaa gtggatatta gcaccgcgga agttgataac cagaagagcc
     4261 aggacctggg ttttatgctg cagaccatcg gcccgaacgt ggatcagcaa attaccctga
     4321 acatcctggc ggagattgcg gacctgaagc gtatgccgaa actggcgcac gatctgcgta
     4381 cctggcaacc gcagccggac ccggtgcaag aacagctgaa gcaactggcg gttgagaaag
     4441 cgcagctgga aaacgaggaa ctgcgtagca aaattcgtct gaacgatgcg caagcgcaga
     4501 aggcgatggc ggagcgtgac aacaaaaacc tggattacct ggagcaggaa agcggtacca
     4561 agcacgcgcg tgacctggag aagatgaaag cgcaaagcca gggcaaccag caactggaaa
     4621 ttaccaaagc gctgaccaag ccgcgtaaag agggtgaact gccgccgaac ctgagcgcgg
     4681 cgattggtta caacgcgctg accaacggtg aagataccgg cattcaaagc gttagcgaac
     4741 gtgacattgc ggcggaagcg aacccggcgt atagcctggg tagcagccaa tttgatccga
     4801 cccgtgatcc ggcgctgaac ccgggtatcc gtctgggtaa ctaatgccag gcatcaaatt
     4861 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     4921 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4981 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5041 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5101 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5161 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5221 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     5281 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5341 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5401 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5461 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5521 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5581 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5641 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5701 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5761 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5821 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5881 ct
//
