LOCUS       sequence-369838-        5186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage Minorna.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_Minorna
FEATURES             Location/Qualifiers
     source          1..5186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..53)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     194..216
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(372..1250)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1277..1561
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1484..1503
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3211..3238
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3257..3348
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3349..3417,3418..4209)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3567..3586)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4380..4968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4869..4888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5122..5139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(5154..5186,1..108)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
       61 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
      121 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      181 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      241 gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa
      301 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
      361 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
      421 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
      481 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
      541 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
      601 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
      661 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
      721 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
      781 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
      841 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
      901 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
      961 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
     1021 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
     1081 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
     1141 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
     1201 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     1261 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     1321 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     1381 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     1441 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     1501 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
     1561 tacccgtttt ttgggctaac aggaggaatt aaccatggat tataagaaaa ccctggcgga
     1621 gctgtttctg ggtgaccagg atgcgggcgt tctggacgcg agcgagaagt tcgcggaatg
     1681 gaccctgccg accatcttta cccgtgaact gtataccgat ggccgtcgta tgcagctgag
     1741 ccgtgactac cagagcaccg gtgcgcaact ggttaacacc gcggcgacca agatcgtggg
     1801 tgcgctgttc ccgcaaggca ccagcttctt tcgttttagc aaaagcgcgg cgctggataa
     1861 cttcattagc agcctgggta gcagcagcac cgcggagagc cgtctgagcg aggttgaaaa
     1921 caccgcgagc cagaaggtgt ttgaaaaaga cggttacgcg gcgaagctgc aagcggttaa
     1981 actgctgctg atcaccggta acgcgctgga gtatattgat gaacgtaccg gcaaaagcat
     2041 catttacagc gttcgtaact tcaccgtgcg tcgtgacggc agcggtaacg tgctgcgtct
     2101 gatcattaag gagcgtgcga gcatccagga cctgccggaa gatattcaaa aagcgcacta
     2161 caacggccgt gatccgtata gcgacatcga tatttacacc gcggcgtgcc gtaagattaa
     2221 acacctgccg aacggcgagg aagcggttag ctacgaggtt tatcaggaag tggacggccg
     2281 tcgtctgggc gagagcagca cctatccgga gctggaactg ccgtacaacg tgctggtttg
     2341 gaacctggtt aacggcgagc actatggccg tggtctggtg gaagactacg cgggtgattt
     2401 tgcgcgtctg agcgtgctga ccgaggcgct gaccaactac gagatcgaag cgagccgtct
     2461 gattccgatg gttgatgcga gcagcggtct ggacgtggat gaatttagcc aagcggagac
     2521 cggtgaagcg gtgcaagttg gtggcggtgg cagcaacggt aacaccaaga gcccggttac
     2581 cgcgtatgag ggtggcgcgg cgcagaaaat ccaatggatt gcgagcgata tccagatgct
     2641 ggaacaaaag ctggcgcgtg cgttcatgta caccggtaac gcgcgtcagg gtgaacgtgt
     2701 gaccgcgtat gaaattcgtc aaaacgcgaa agaggcggaa tacgcgatgg gtggcggttt
     2761 tagcatcctg agcgacagct ggctgcgtaa actggcgtac ctgtatacca ttgttgcgta
     2821 tccgaagttc aaagtgtacc tgaccgaggg tgtggttgat atcaacgtga tggttggcac
     2881 cgcggcgctg gcgaaagcgg cggcggcgga caaactgctg gaggcgaccc aggcgatgca
     2941 actggcgatc ccggttctga agcagctgac cccgcgtttt aacgaagacg cgtgcgttga
     3001 ttggtacctg gacgcgtatg gcattgtgag cggtccgttc atgctgaccg aggaacagct
     3061 gcaagaaaaa caacaggtgc aggacaacgc ggcggcggcg agcgcggcgc aggcgcaaag
     3121 ccaggttcaa gatctgcaag cggcggaccc gaccgtggcg ggtcagcaac tgggcctgct
     3181 gccgggctaa tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     3241 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3421 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3481 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3541 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     3601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3661 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3721 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3781 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3901 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4081 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4141 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4201 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4501 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5041 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5101 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5161 cggtattttc tccttacgca tctgtg
//
