LOCUS       sequence-369843-        5183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage ECBP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_ECBP5
FEATURES             Location/Qualifiers
     source          1..5183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..63
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    78..218
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(144..163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     304..326
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(482..1360)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1387..1671
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1594..1613
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3318..3345
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3364..3455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3456..3524,3525..4316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3674..3693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4487..5075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4976..4995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
       61 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      121 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
      181 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
      241 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      301 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
      361 agatcaattc gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa atggacgaag
      421 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
      481 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
      541 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
      601 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
      661 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
      721 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
      781 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg
      841 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
      901 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
      961 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa
     1021 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
     1081 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga
     1141 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct
     1201 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
     1261 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
     1321 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
     1381 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
     1441 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
     1501 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
     1561 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
     1621 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
     1681 ttgggctaac aggaggaatt aaccatgcgt ggctacagcc cgatcattac caagggtggc
     1741 gacgtgccgt atatgaacaa ccgtatgaag gttaaagagg aaagcggcaa gccgaaaagc
     1801 accctggcgg agcgttacaa gaccctgagc gcgaaacgtg aaacccaact gagccgtgcg
     1861 aagatgtatg cgaaaatcac cctgccggcg ctgttcaccg aggatcaaag caccgaccag
     1921 ggcgatgcgt acacccaaaa cagctggcaa agcctgggtg cgcagggcgt gaaccacctg
     1981 gcgaacaaac tggttatgac ctggttcccg ccgcaacgta gcttctttaa gctggagttt
     2041 accgacgaag cgaaacaggc gctgtatgat accggtaccg acgatgtgga actgctgagc
     2101 attatggcgc gtgcggagaa caaggcgcgt ctgctgcacg aacaaattca aggtcgtatt
     2161 gcgtggaccc aggcggcgaa acacctgatc gttgcgggta acgcgatgct gtacgcgccg
     2221 gtgaacgaca gcctggtttg ctacccgatg gataagtatg tggttaagcg tagcaaaacc
     2281 ggtcgtgtga cccaaatgat tctggaggaa gttaaatgcc tgagcgagtt cccgccggcg
     2341 gtgcaggcga tcattaaggc gaaacgtttt aacctgaagc aagacgatac cgttgaactg
     2401 tacacccagg cgaaatggga cggcagcatg tatgtgatta cccaggaagc ggttgatacc
     2461 accatcggca aggaataccg tgtgaaaccg gaggaaagcc cgttcatcgt tctgacctgg
     2521 gagcgtctgt acggtgaaga ctatggtcgt ggcctggtgg agagctgcta cggcgacctg
     2581 ttcacctatg cgtttctgag caaggcggtt gcgaaaggtt gcgcgctgat gagcgaggtg
     2641 aagtttctgg ttaaacgtgg cagcgcgacc agcccgaccg cgcatgcgaa ggcggaaacc
     2701 ggtgactacg tgtggggtga ggaaggcgat gttagcgtgg ttcaactgga gaagtacggc
     2761 gactatcaga ccgtgctgag cgttctggaa acctacagca aacgtctggg tcaagcgttc
     2821 ctgctggcga gcgcgaaccg tcgtgatgcg gagcgtgtga ccacctatga actgcgtatg
     2881 gatgcgatgg agctggaaac cagcctgggt ggcacctaca gccaaattgc tgtgagcggt
     2941 cagctgccgt atgcgaacct gctgctgcag cgtagcggtt ttaagctgcc ggcgaccgat
     3001 gcggttccga tcattatcac cggtattgag gcgctgggca aggcgggtga actggataaa
     3061 ctgatgcaac tgagcgagat gatggcgatt ccgaacagct ggagcaccgc ggcgcaggaa
     3121 cgtatcaaat ggagcgacta cattgcgttt atcgcggcga acctgaacat ggagaccagc
     3181 tggctgatga ccgaggatga atataacaag atgaaacagg cgcagcaaca gctgatgcaa
     3241 cagcaacaac tgatggaagc ggcgggcaag gcggcgccgc aaatgatgaa acagtaatgc
     3301 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     3361 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3421 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3481 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3541 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3601 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3661 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     3721 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3781 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3841 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3901 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3961 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4621 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5161 ctgtggataa ccgtattacc gcc
//
