LOCUS       sequence-369844-        9246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NFAT - PKC/Ca++ gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE17
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1946..1975
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1976..2005
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    2006..2035
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        2078..2109
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2136..2145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2142..3791
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3795..3914
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4006..4594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4059..4079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4477..4488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4609..4925
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4776..4911
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4976..4978,4979..4981,4982..5695)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5021..5042)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5282..5301)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5629..5650
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5767..6000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6072..6193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6109..6128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6163..6182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6233..6368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6388..6407)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6389..6407)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6417..6434)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6417..6433)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6426..6448)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6575..7030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6662..6681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6872..6893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7056..7160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7161..7229,7230..8021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7379..7398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8192..8780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8681..8700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8934..8951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9068..9089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9104..9109,9110..9127,9128..9134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9142..9158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9147..9169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9166..9182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9166..9182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9201..9221
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9203..9221
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcggagg aaaaactgtt tcatacagaa ggcgtggagg
     1981 aaaaactgtt tcatacagaa ggcgtggagg aaaaactgtt tcatacagaa ggcgtgatcc
     2041 gagaacagat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
     2101 gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca
     2161 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     2221 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     2281 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     2341 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     2401 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     2461 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     2521 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     2581 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     2641 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     2701 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     2761 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     2821 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     2881 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     2941 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     3001 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     3061 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     3121 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     3181 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     3241 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     3301 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     3361 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     3421 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     3481 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     3541 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     3601 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     3661 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     3721 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     3781 agatcgccgt gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc
     3841 tgcccatgag ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg
     3901 ccaggatcaa cgtctaaggc cgcgactcta gaacgcgtca ctgcggctta ccggtcatga
     3961 agtcggacta cagacatcag cgcgtaccgg ttagtaatga tcgacaatca acctctggat
     4021 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4081 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4141 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     4201 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     4261 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     4321 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4381 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     4441 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4501 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4561 acgagtcgga tctccctttg ggccgcctcc ccgcctggcg atggtaccgg tgtggaaagt
     4621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4681 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4741 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4861 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4921 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac ctagcatggt
     4981 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5041 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     5101 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5161 gaccaccttc acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     5221 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     5281 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5341 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     5401 ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat
     5461 caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     5521 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5581 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5641 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggtac
     5701 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5761 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5821 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5881 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5941 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6001 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6061 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6121 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6181 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6361 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6421 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6481 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6541 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6601 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6661 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6721 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6781 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6841 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6901 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6961 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7021 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8881 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8941 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9001 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9061 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9181 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     9241 aagctt
//
