LOCUS       sequence-369845-        5156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage Lidtsur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_Lidtsur
FEATURES             Location/Qualifiers
     source          1..5156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..38
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    53..193
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(119..138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     279..301
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(457..1335)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1362..1646
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1569..1588
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3266..3293
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3312..3403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3404..3472,3473..4264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3622..3641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4435..5023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4924..4943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
       61 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      121 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
      181 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      241 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      301 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa
      361 gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
      421 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
      481 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
      541 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
      601 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
      661 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
      721 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
      781 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
      841 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
      901 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
      961 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
     1021 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
     1081 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
     1141 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
     1201 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     1261 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     1321 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     1381 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     1441 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     1501 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     1561 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     1621 atcgcaactc tctactgttt ctccataccc gttttttggg ctaacaggag gaattaacca
     1681 tggtgcagga cgcgcaaaac gttagctacg agagcctgtg gcagaagtat cgtgatagca
     1741 acgtggttac caaagcgcgt gagtacagcc gttataccct ggcgaagctg gttagcgaat
     1801 acgaccgtag cgatgtggac gatgttaacc gtcagcaagt gacccgtgac tatcaaagcg
     1861 ttggtgcgct gctggtgaac aacctggttg cgcgtctggg cgaattcctg tttccggcga
     1921 gcgcgcgttt cgtgaagctg aaactgaaca aggttaccga tgcgatgcgt cagaaaatga
     1981 gccaggtgaa ccaagcgctg atcaccattg agaaagcgat cgttgacaag gcgaaagaaa
     2041 acggtggcta cgcggatctg ctgcaaaccc tggcgcagca agcggtgacc ggtaacgcgg
     2101 cgctgtatcg tgacatggag accggtagct accgtgttta tggcctggaa agctttgtgg
     2161 ttcagcgtga cggtcgtggc gtggttaccg atgcgatcat taaggagcgt ctgcagtggg
     2221 acagcctgcc gaaagatttc caaaacatgc tgaacgcgaa gggttttcgt tgcggtggca
     2281 acaaacgtgt gtggctgtac acccgtgtgc tgcgtgttca gaaggagagc ggtaaatacg
     2341 gctatgaaat cacccagcaa attggtaact accaaggctg cgtttatacc ccgggcaagg
     2401 actactatcc ggaaaaagtg tgcccgtggc tgttcccggt ttggagcctg aagagcggcg
     2461 agcactacgg tcgtggcatc gtggaagacc acgcgggcga ttttgcgcgt ctgagcctgc
     2521 tgagcgagca cagcgcgctg tatatgcagg aagcgctgcg tgttctgtgg ctgatgaccg
     2581 gtagcggtgg caacgcggac gatattgaga acgcggaaac cggtcaggtg gtgccgctgc
     2641 aaagcggtac caccctggcg ggtctggaag tgggcgacta caacaaggtt cagcaagcgc
     2701 gtgatgaaat caaccagatt gtgcaacgtc tggcgcaggc gttcatgtat accggcgagt
     2761 ttcgtgacag cgaacgtacc accgcgaccg aggtgcagca agttgcgacc agcgcggaac
     2821 gtggcctggg tggcccgtac agcatgcagg cgaaaaccct gcaaatcccg ctggcgtatg
     2881 tgctgctggc ggagctggat gaagcgaacg ttccggacat tgtgggtgat gttctgagcc
     2941 tgcaagttga agcgggtctg gatgcgctgg gccgtagcat tgaggcggaa accctgattc
     3001 gtgcgctgac cgatagccag gcggcgatgg tggcggttca aaacatcaac accgtggcga
     3061 acgttattga cccgcagcaa gtgctggaga ccatcttcgc gagcaacggt gttgcgctgg
     3121 acgattaccg taaaagcgcg caggacctgg cgaacgaagg ccaagcgatc gataacgcgg
     3181 cggcggcggc ggcgggtgtg caaccgggcg tggttgatac cgcgccgaac gttagccaaa
     3241 tttaatgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     3301 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     3661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4561 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4981 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgcc
//
