LOCUS       sequence-369850-        4173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A5_STOP
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1730..1829)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1846..1865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1847..1865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1870..1886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1870..1886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1988..2007)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1999..2808
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2066..2085)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2955..3543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3444..3463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3697..3714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3873..3900)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3992..4078)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4127..4149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4141..4158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4142..4158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgat gggatctccc
      121 gtgagccacc tgctggcagg attctgcgtg tgggtggtgc tgggatgggt gggaggatcc
      181 gtgccaaatc tgggacctgc agagcaggag cagaaccact acctggccca gctgtttggc
      241 ctgtatggag agaatggcac cctgacagca ggaggactgg caaggctgct gcactctctg
      301 ggcctgggcc gggtgcaggg cctgagactg ggccagcacg gaccactgac cggaagggca
      361 gcatcccctg ccgccgataa ttctacacac cgcccccaga accctgagct gagcgtggac
      421 gtgtgggcag gaatgccact gggaccaagc ggctggggcg atctggagga gtccaaggca
      481 cctcacctgc caaggggacc agcacccagc ggcctggacc tgctgcacag actgctgctg
      541 ctggaccact ccctggccga tcacctgaac gaggactgcc tgaatggctc ccagctgctg
      601 gtgaacttcg gactgtctcc agcagcacct ctgaccccaa ggcagtttgc cctgctgtgc
      661 cctgccctgc tgtaccagat cgacagccgg gtgtgcatcg gagcacctgc accagcacca
      721 cctggcgatc tgctgtctgc cctgctgcag agcgccctgg ccgtgctgct gctgtccctg
      781 ccatctcctc tgagcctgct gctgctgagg ctgctgggac ctaggctgct gaggccactg
      841 ctgggcttcc tgggcgccct ggcagtgggc accctgtgcg gcgacgccct gctgcacctg
      901 ctgcctcacg cacaggaggg cagacacgca ggaccaggag gactgcccga gaaggatctg
      961 ggaccaggac tgtccgtgct gggaggactg ttcctgctgt ttgtgctgga gaatatgctg
     1021 ggactgctga ggcacagggg actgaggccc agatgctgtc ggagaaagag gcgcaacctg
     1081 gagacccgga atctggaccc agagaacggc tctggcatgg ccctgcagcc actgcaggca
     1141 gcaccagagc caggagcaca gggacagaga gagaagaatt ctcagcaccc acccgccctg
     1201 gcacctccag gacaccaggg acacagccac ggccaccagg gcggcaccga tatcacatgg
     1261 atggtgctgc tgggcgatgg cctgcacaac ctgacagacg gactggcaat cggagcagcc
     1321 ttctctgacg gctttagctc cggcctgagc accacactgg ccgtgttttg tcacgagctg
     1381 ccacacgagc tgggcgattt cgccatgctg ctgcagtccg gcctgtcttt tcggagactg
     1441 ctgctgctga gcctggtgtc cggcgccctg ggactgggag gagccgtgct gggcgtggga
     1501 ctgtccctgg gacctgtgcc actgacccca tgggtgttcg gagtgacagc aggcgtgttt
     1561 ctgtatgtgg ccctggtgga tatgctgcct gccctgctga ggccacctga gcccctgcct
     1621 accccacacg tgctgctgca gggactggga ctgctgctgg gaggaggact gatgctggca
     1681 atcacactgc tggaggagcg cctgctgcca gtgaccacag agggctaaga cccagctttc
     1741 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1801 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1861 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1921 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1981 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2041 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2101 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2161 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2221 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2281 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2341 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2401 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2461 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2521 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2581 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2641 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2701 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2761 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2821 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2881 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3301 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3361 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3421 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3481 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3541 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3601 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3661 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3721 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3781 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3841 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3901 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3961 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4021 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4081 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4141 tgtaaaacga cggccagtct taagctcggg ccc
//
