LOCUS       sequence-369852-        5138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage SP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_SP6
FEATURES             Location/Qualifiers
     source          1..5138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..75
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    90..230
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(156..175)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     316..338
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(494..1372)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1399..1683
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1606..1625
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3285..3312
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3331..3422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3423..3491,3492..4283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3641..3660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4454..5042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4943..4962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
       61 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
      121 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      181 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
      241 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      301 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      361 gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa tgtgcctgtc
      421 aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct
      481 gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
      541 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
      601 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
      661 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
      721 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
      781 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
      841 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
      901 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
      961 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc
     1021 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
     1081 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
     1141 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
     1201 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
     1261 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
     1321 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
     1381 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
     1441 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
     1501 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
     1561 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     1621 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     1681 catacccgtt ttttgggcta acaggaggaa ttaaccatgc aggacaccat cctggaatac
     1741 ggtggccaac gtagcaagat tccgaaactg tgggagaagt tcagcaagaa acgtagcccg
     1801 tacctggacc gtgcgaagca ctttgcgaaa ctgaccctgc cgtatctgat gaacaacaag
     1861 ggtgataacg aaaccagcca aaacggttgg caaggtgtgg gtgcgcaggc gaccaaccac
     1921 ctggcgaaca aactggcgca ggttctgttc ccggcgcaac gtagcttctt tcgtgtggat
     1981 ctgaccgcga agggtgaaaa agttctggac gatcgtggcc tgaagaaaac ccaactggcg
     2041 accatctttg cgcgtgtgga aaccaccgcg atgaaggcgc tggagcagcg tcaattccgt
     2101 ccggcgatcg ttgaggtgtt taaacacctg attgtggcgg gtaactgcct gctgtacaag
     2161 ccgagcaaag gcgcgatgag cgcggttccg atgcaccact atgtggttaa ccgtgacacc
     2221 aacggtgacc tgatggatgt tatcctgctg caggaaaaag cgctgcgtac ctttgatccg
     2281 gcgacccgta tggcgattga agtgggtatg aagggcaaga aatgcaaaga ggacgataac
     2341 gttaaactgt acacccacgc gcagtatgcg ggcgagggct tctggaagat caaccaaagc
     2401 gcggacgata ttccggtggg taaagaaagc cgtatcaaga gcgagaaact gccgtttatt
     2461 ccgctgacct ggaaacgtag ctatggcgag gactggggtc gtccgctggc ggaggactat
     2521 agcggcgacc tgttcgttat ccagtttctg agcgaagcga tggcgcgtgg tgcggcgctg
     2581 atggcggata tcaagtacct gattcgtccg ggcagccaaa ccgacgtgga tcacttcgtt
     2641 aacagcggta ccggcgaagt gatcaccggt gttgcggagg acatccacat tgtgcagctg
     2701 ggcaaatacg cggatctgac cccgattagc gcggtgctgg aagtttatac ccgtcgtatc
     2761 ggtgttattt ttatgatgga gaccatgacc cgtcgtgatg cggaacgtgt gaccgcggtt
     2821 gagatccagc gtgatgcgct ggaaattgag caaaacatgg gtggcgtgta tagcctgttc
     2881 gcgatgacca tgcagacccc gatcgcgatg tggggtctgc aagaagcggg cgacagcttt
     2941 accagcgagc tggttgatcc ggtgatcgtt accggtattg aagcgctggg ccgtatggcg
     3001 gagctggaca agctggcgaa cttcgcgcag tacatgagcc tgccgcaaac ctggccggag
     3061 ccggcgcagc gtgcgatccg ttggggtgac tatatggatt gggtgcgtgg ccaaattagc
     3121 gcggaactgc cgtttctgaa aagcgaggaa gagatgcagc aagagatggc gcagcaagcg
     3181 caggcgcagc aagaagcgat gctgaacgag ggtgttgcga aggcggtgcc gggcgttatt
     3241 cagcaagaaa tgaaagaggg ttaatgccag gcatcaaatt aagcagaagg ccatcctgac
     3301 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     3361 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3421 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3481 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     3541 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     3601 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     3661 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3721 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     3781 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3841 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3901 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3961 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4021 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4081 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4141 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4201 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4261 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4321 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4381 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgttctttcc tgcgttatcc cctgattctg tggataac
//
