LOCUS       sequence-369853-        3936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A6_STOP
FEATURES             Location/Qualifiers
     source          1..3936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1493..1592)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1609..1628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1610..1628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1633..1649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1633..1649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1751..1770)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1762..2571
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1829..1848)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2718..3306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3207..3226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3460..3477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3636..3663)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3755..3841)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3890..3912
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3904..3921
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3905..3921
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg caccatccac
      121 ctgttcagga agccccagcg gagcttcttc ggcaagctgc tgcgggagtt tagactggtg
      181 gccgccgacc ggagatcttg gaagatcctg ctgttcggcg tgatcaatct gatctgcaca
      241 ggctttctgc tgatgtggtg tagctccacc aacagcatcg ccctgaccgc ctacacatat
      301 ctgaccatct tcgatctgtt tagcctgatg acatgcctga tctcctactg ggtgaccctg
      361 aggaagccat ctcccgtgta tagcttcggc tttgagcgcc tggaggtgct ggccgtgttc
      421 gcatctaccg tgctggcaca gctgggcgcc ctgttcatcc tgaaggagag cgccgagcgg
      481 tttctggagc agccagagat ccacacaggc agactgctgg tgggcacctt cgtggccctg
      541 tgcttcaacc tgtttacaat gctgtctatc cggaataagc ccttcgccta cgtgagcgag
      601 gcagcatcta ccagctggct gcaggagcac gtggcagacc tgtccaggtc tctgtgcggc
      661 atcatccctg gcctgtctag catcttcctg cctcgcatga atccatttgt gctgatcgac
      721 ctggcaggag ccttcgccct gtgcatcaca tacatgctga tcgagatcaa caattatttt
      781 gccgtggata ccgccagcgc catcgccatc gccctgatga cattcggcac catgtaccca
      841 atgagcgtgt attccggcaa ggtgctgctg cagaccacac cccctcacgt gatcggccag
      901 ctggacaagc tgatccggga ggtgtccaca ctggatggcg tgctggaggt gagaaacgag
      961 cacttctgga ccctgggatt tggatccctg gcaggatctg tgcacgtgcg gatcaggcgc
     1021 gacgccaacg agcagatggt gctggcccac gtgacaaata gactgtacac cctggtgagc
     1081 accctgacag tgcagatctt taaggacgat tggatcaggc ccgccctgct gtccggacct
     1141 gtggcagcca acgtgctgaa tttctctgat caccacgtga tccctatgcc actgctgaag
     1201 ggcacagacg atctgaatcc tgtgaccagc acaccagcca agccctcctc tccaccccct
     1261 gagttctcct ttaacacccc aggcaagaac gtgaatcccg tgatcctgct gaacacccag
     1321 acacgccctt acggctttgg cctgaatcac ggccacaccc catatagctc catgctgaac
     1381 cagggactgg gagtgccagg aatcggagcc acacagggcc tgaggacagg cttcaccaat
     1441 atcccttccc gctatggcac caacaatcgg atcggccagc ccagacctta agacccagct
     1501 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1561 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1621 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1681 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1741 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1801 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1861 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1921 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1981 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2041 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2101 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2161 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2221 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2281 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2341 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2401 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2461 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2521 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2581 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2641 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3361 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3421 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3481 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3541 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3601 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3661 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3721 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3781 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3841 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3901 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
