LOCUS       sequence-369854-        5144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage PGT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_PGT2
FEATURES             Location/Qualifiers
     source          1..5144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..34
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    49..189
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(115..134)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     275..297
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(453..1331)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1358..1642
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1565..1584
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3250..3277
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3296..3387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3388..3456,3457..4248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3606..3625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4419..5007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4908..4927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
       61 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      121 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
      181 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      241 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      301 tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg
      361 catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
      421 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
      481 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
      541 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
      601 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
      661 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
      721 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
      781 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
      841 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
      901 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
      961 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     1021 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     1081 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     1141 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     1201 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     1261 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     1321 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     1381 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     1441 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     1501 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     1561 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     1621 caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat taaccatgac
     1681 caacgcggat aacgcggtgc aaagcaccgt taccgcgccg agccgttgga ccgaactgga
     1741 tggtcagcgt cgtagcctgg tgcgtcgttg ggaaagctac agcatctata ccattccgta
     1801 catctgcccg gatgagaact atcagcaaga taccgacgaa atgacccacg actttcaagc
     1861 ggtgggtgcg caggcggtta acagcctggc gaacaagatt atgctggcgc tgtttgcgcc
     1921 gagccgtccg ttctttaaac tggacccgga cgatgacacc aaagaggagc tgatgaaagt
     1981 gggtatcgat ccggacgcgc tgaaggatgt ttttagcagc ctggagctgc agagcgcgaa
     2041 gctgctggac accaaaaact tccgtccggt gatgtttgaa atcattaaga gcctgattgt
     2101 taccggcaac agcctgatgg atctgggtaa cgaggttgcg tacgtgcgtg ttcgtgattt
     2161 tgtggttaaa cgtgactact atggtcgtcc gtgcgaaatc atgattaaga cctgcctgaa
     2221 attcgagcaa ctgaccatgg acgtgcagga aagctgccag caaaagaaac cgcacggcta
     2281 caccccggat accaaggtgg acgtttaccg ttggtataaa gttgatgaga ccggcaagcg
     2341 tatgaaaatg acctactgga tcgatgacct gagcctggag gacggcaagt atcagggtga
     2401 ttggaaacgt gaagattgcc cggtgcgtcc gctgacctgg tgcctgaccg cgaacgcgga
     2461 ttacggtacc ggcctggttg aggaatatgc gggtgacttt gcggcgctga gcaccatgag
     2521 caccagcttt gtgcaaggta gcgttctggc gagcgagttc cgttggctga gcaacccggc
     2581 gggtatgacc cagccggaag atgtgcgtga cagcaacaac ggtgcggtga ttccgggcgt
     2641 tgaaggtgat ctgagcctga tcagcgcggg tcaaggcatg ggtcaaaccc tgcagatgat
     2701 gatgaacggt atggatattt acacccgtcg tatcagccag ggcttcctga tgatgagcgg
     2761 tgtgacccgt gatgcggagc gtgttaccgc ggaggaaatt cgtatgcagg cgaacgagct
     2821 ggaaaacagc ctgggtggcg cgtatagccg tctggcgatt gacatgcaac tgccgatcgc
     2881 gaactggctg ctggatatgc aggacctgag cattaagggc gttaaaatga acgtgagcat
     2941 cgttaccggc ctgaacgcgc tgagccgtaa cggtgatctg gacaacctga acgcggcggt
     3001 gggtaacatc accgcgatgt accaaatgcc gccgctggcg atgcgtctgc tgaagatgga
     3061 tgcggttatt agcgacatta tggcgggtca tggtctgctg gcggcgcgtt atgtgaaaac
     3121 ccaagcggaa gttgcgcagg aagagcagca agcgcaacag caagcgctgg cgcaacaggg
     3181 tgcggcgaac gtggttgacc aggcgaccat gccgcaacag ccgatgtaat gccaggcatc
     3241 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     3301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3601 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4561 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5101 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcag
//
