LOCUS       sequence-369855-        5159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage Pisces.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_Pisces
FEATURES             Location/Qualifiers
     source          1..5159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..34
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    49..189
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(115..134)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     275..297
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(453..1331)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1358..1642
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1565..1584
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3265..3292
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3311..3402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3403..3471,3472..4263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3621..3640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4434..5022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4923..4942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
       61 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      121 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
      181 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      241 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      301 tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg
      361 catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
      421 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
      481 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
      541 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
      601 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
      661 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
      721 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
      781 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
      841 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
      901 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
      961 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     1021 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     1081 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     1141 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     1201 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     1261 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     1321 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     1381 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     1441 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     1501 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     1561 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     1621 caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat taaccatggc
     1681 ggagcgtcaa ggttttgcgg cggaaggtgc gaaggcggtt tatgaccgtc tgaaaaacgg
     1741 ccgtcaaccg tatgaaaccc gtgcgcaaaa ctgcgcggcg gtgaccatcc cgagcctgtt
     1801 tccgaaggag agcgataaca gcagcaccga atataccacc ccgtggcagg cggttggtgc
     1861 gcgttgcctg aacaacctgg cggcgaagct gatgctggcg ctgttcccgc aagcgccgtg
     1921 gatgcgtctg accgttagcg agtatgaagc gaaaaccctg agccaggaca ccgaagcggc
     1981 ggcgcgtgtg gatgagggtc tggcgatggt ggaacgtgtt ctgatggcgt acatggagac
     2041 caacagcttc cgtgtgccgc tgtttgaagc gctgaaacag ctgatcgtta gcggcaactg
     2101 cctgctgtat atcccgccgc cggagcaggg tcaatacagc ccgatgcgta tgtatcgtct
     2161 ggttagctac gtggttcaac gtgacgcgtt cggtaacgtg ctgcagatcg ttaccctgga
     2221 ccgtattgcg tttagcgcgc tgccggaaga tgtgcagagc caactgaacg cggacgatta
     2281 cgagccggac accgaactgg atgtttacac ccacatctat cgtgagggcg atgaatacct
     2341 gcgttatgag gaagtggagg gtgtggaagt taacggcacc gacggtagct atccgctgga
     2401 tgcgtgcccg tacattccgg tgcgtatggt tcgtctggac ggcgaggatt acggtcgtag
     2461 ctatgcggag gaatacctgg gtgacctgaa cagcctggag accatcaccg aagcgattac
     2521 caagatggcg aaagtggcgg cgaaggtggt tggcctggtt aacccgaacg gtatcaccca
     2581 accgcgtcgt ctgaacaaag cggcgaccgg cgagtttgtt gcgggtcgta tcgaagatat
     2641 taacttcctg cagctgacca agggccaaga ctttaccatc gcgaaaagcg tggcggatgc
     2701 gattgagcaa cgtctgggtt gggcgttcct gctgaacagc gcggtgcagc gtaacgcgga
     2761 acgtgttacc gcggaggaaa tccgttatgt tgcgggtgaa ctggaagcga ccctgggtgg
     2821 cgtgtacagc gttcagagcc aagagatgca gctgccgctg gtgcgtgttc tgctgaacca
     2881 gctgcaaagc gcgggcatga ttccggacct gccgaaagag gcggtggaac cgaccgttag
     2941 caccggtctg gaggcgctgg gtcgtggtca agatctggaa aaactgaccc aggcggtgaa
     3001 catgatgacc ggtctgcagc cgctggcgca agacccggat atcaacctgc cgaccctgaa
     3061 gctgcgtctg ctgaacgcgc tgggcattga caccgcgggt ctgctgctga cccaggatga
     3121 gaaactgcaa cgtatggcgg aacagagcag ccagcaagcg gtggttcagg gtgcgggtgc
     3181 ggcgggtgcg aacatgggtg cggcggttgg tcagggtgcg ggtgaagaca tggcgcaggc
     3241 gtaatgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     3301 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     3661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3781 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3841 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3901 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3961 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4021 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4081 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4141 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4201 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4261 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4321 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4381 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4441 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4501 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4561 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4621 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4681 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4741 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4801 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4861 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4921 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4981 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5041 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5101 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcag
//
