LOCUS       sequence-369856-        4848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A5_STOP
FEATURES             Location/Qualifiers
     source          1..4848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2405..2504)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2521..2540)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2522..2540)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2545..2561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2545..2561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2663..2682)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2674..3483
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2741..2760)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3630..4218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4119..4138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4372..4389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4548..4575)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4667..4753)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4802..4824
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4816..4833
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4817..4833
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggagaagtac
      121 ggcggcgacg tgctggcagg cccaggagga ggaggaggac tgggccctgt ggatgtgcca
      181 tccgcccggc tgaccaagta catcgtgctg ctgtgcttca caaagtttct gaaggccgtg
      241 ggcctgttcg agtcttatga cctgctgaag gccgtgcaca tcgtgcagtt catctttatc
      301 ctgaagctgg gcaccgcctt ctttatggtg ctgttccaga agccttttag ctccggcaag
      361 accatcacaa agcaccagtg gatcaagatc tttaagcacg ccgtggccgg ctgcatcatc
      421 tctctgctgt ggttctttgg actgaccctg tgcggaccac tgcggacact gctgctgttc
      481 gagcacagcg atatcgtggt catctccctg ctgtctgtgc tgtttacctc tagcggagga
      541 ggaccagcaa agacaagagg agccgccttc tttatcatcg ccgtgatctg cctgctgctg
      601 ttcgacaacg acgatctgat ggccaagatg gccgagcacc ctgagggcca ccacgattcc
      661 gccctgaccc acatgctgta tacagcaatc gccttcctgg gagtggcaga ccacaaggga
      721 ggcgtgctgc tgctggtgct ggccctgtgc tgtaaagtgg gctttcacac cgcctcccgg
      781 aagctgtctg tggatgtggg aggagcaaag agactgcagg ccctgagcca cctggtgtcc
      841 gtgctgctgc tgtgcccctg ggtcatcgtg ctgtccgtga ccacagagtc taaggtggag
      901 agctggttct ccctgatcat gccttttgcc acagtgatct tctttgtgat gatcctggac
      961 ttctacgtgg attctatctg cagcgtgaag atggaggtga gcaagtgtgc ccggtatggc
     1021 tctttcccaa tctttatcag cgccctgctg ttcggcaact tttggaccca ccccatcaca
     1081 gaccagctga gagccatgaa taaggcagca caccaggagt ccaccgagca cgtgctgtct
     1141 ggaggagtgg tggtgagcgc catcttcttt atcctgagcg ccaacatcct gtcctctcct
     1201 tccaagcggg gccagaaggg caccctgatc ggatactccc cagagggcac acctctgtat
     1261 aatttcatgg gcgacgcctt tcagcacagc tcccagtcta tcccaaggtt catcaaggag
     1321 agcctgaagc agatcctgga ggagtccgat tctcgccaga tcttctactt tctgtgcctg
     1381 aacctgctgt tcacctttgt ggagctgttt tatggcgtgc tgacaaatag cctgggcctg
     1441 atctccgacg gcttccacat gctgtttgat tgtagcgccc tggtcatggg actgttcgcc
     1501 gccctgatgt ccaggtggaa ggcaaccaga atcttttctt acggctatgg caggatcgag
     1561 atcctgtctg gcttcatcaa cggcctgttt ctgatcgtga tcgccttctt cgtgttcatg
     1621 gagagcgtgg caaggctgat cgacccacct gagctggata cccacatgct gacaccagtg
     1681 tccgtgggcg gcctgatcgt gaatctgatc ggcatctgcg ccttttctca cgcacacagc
     1741 cacgcacacg gagcatccca gggctcttgt cactctagcg accacagcca ctcccaccac
     1801 atgcacggac actccgatca cggacacgga cacagccacg gatccgccgg aggaggcatg
     1861 aacgccaata tgaggggcgt gttcctgcac gtgctggcag acaccctggg atccatcggc
     1921 gtgatcgtgt ctacagtgct gatcgagcag ttcggctggt ttatcgccga ccctctgtgc
     1981 agcctgttca tcgccatcct gatctttctg tccgtggtgc cactgatcaa ggatgcctgt
     2041 caggtgctgc tgctgaggct gccacccgag tacgagaagg agctgcacat cgccctggag
     2101 aagatccaga agatcgaggg cctgatcagc tatagggatc cccacttctg gaggcactct
     2161 gccagcatcg tggcaggcac catccacatc caggtgacat ccgacgtgct ggagcagcgc
     2221 atcgtgcagc aggtgaccgg catcctgaag gatgccggcg tgaacaatct gacaatccag
     2281 gtggagaagg aggcctactt ccagcacatg tctggcctga gcaccggctt tcacgacgtg
     2341 ctggccatga caaagcagat ggagagcatg aagtactgta aggatggcac ctatatcatg
     2401 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2461 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     2521 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     2581 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2641 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2701 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2761 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2821 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2881 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2941 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     3001 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     3061 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     3121 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     3181 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     3241 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     3301 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3361 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3421 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3481 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3541 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4501 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     4561 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     4621 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     4681 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     4741 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     4801 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
