LOCUS       sequence-369857-        5147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from uncultured Caudovirales phage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_uncultured_phage
FEATURES             Location/Qualifiers
     source          1..5147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     405..424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     658..675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    690..830
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(756..775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     916..938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1094..1972)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1999..2283
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2206..2225
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3894..3921
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3940..4031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4032..4100,4101..4892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4250..4269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5063..5147,1..504)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
       61 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      541 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      601 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      661 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
      721 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
      781 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
      841 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      901 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      961 gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa tgtgcctgtc
     1021 aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct
     1081 gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
     1141 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
     1201 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
     1261 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
     1321 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
     1381 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
     1441 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
     1501 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
     1561 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc
     1621 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
     1681 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
     1741 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
     1801 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
     1861 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
     1921 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
     1981 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
     2041 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
     2101 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
     2161 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     2221 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     2281 catacccgtt ttttgggcta acaggaggaa ttaaccatga acggtagcgc ggcgcacctg
     2341 tatagcgagc tggaaaccca acgtgacagc tacctgctgc gtggtcgtga ttgcagccgt
     2401 ctgaccctgc cgaccctgct gccggaccaa ggtaccagcg aggtgaccaa atttccgacc
     2461 ccgtttcagg gtatgggtgc gcgtggtgtt aaccacctgg cggcgagcct gctgatgagc
     2521 ctgctgccgc cgaaccagcc gttctttcgt ctgatcctgg acgaggaagc gattcgtgcg
     2581 ctgggcgagg cggaggaata taaaaccgaa atcgatcaaa ccctgagcag cattgaacgt
     2641 gcggtgatgc aggagatcga aaccatggcg attcgtagcg cggttttcga ggcgctgaag
     2701 cacctgatcg tgaccggtaa cgcgctgatt tatctgggtg aagacggcat ccgtgtgttt
     2761 cacctggacc gttacgtggt taaacgtgat ccgagcggca aagttctgaa gattctggtg
     2821 aaggagaccg ttagcccgat cgaactgccg gaggaagcgc aaccgctggt gcagaacaaa
     2881 cgtgcgattg acgataccgt tgatctgtac acctgcatcc acaacaccgg taacggccgt
     2941 tacgaggcgt atcaagaagt tatgggtcag cgtctgccga gcagctacgg cagctataaa
     3001 gaggacagca tgccgtggct ggcgctgcgt atgaaccgtg tggatggtga aagctacggt
     3061 cgtggctatg ttgaggaata cctgggtgac ctgcgtagcc tggagggtct gacccaagcg
     3121 attgtggaag gtagcgcggc ggcggcgaaa gtgctgttcc tggttaaccc gaacggcatg
     3181 acccgtgcgg atgttctggc gaagagcccg aacggtgcga tccgtgaggg catggcgagc
     3241 gacgtgagca ccctgcagct gcaaaaacag gcggatttcg gtgttgcgct gcaagcgatc
     3301 agcggcattc gtgaacgtct gaactatgcg tttctgctgg cggagagcac cattcgtaac
     3361 gcggaacgtg tgaccgcgga ggaagttcgt ctgaccaccg cggcggtgga gcgtcagctg
     3421 ggtggcatct acagcattct ggcgcaagaa ttccagctgc cgctggttaa ccgtctgatg
     3481 gacagcatga gccgtaagaa aaagatgccg cgtgtgccga aagagtttgt taagccgatg
     3541 atcgtgaccg gtattgatgc gctgggtcgt ggcaacgacc tggttaaact ggatgcgctg
     3601 ctggcgggtg tggcgcagac ctttggtccg caagcggttg cgcagtatat caacgcgggt
     3661 gaatacctgg cgcgtcgtgc ggcggcgctg ggtatcgaca ccaaaggcct gattaagagc
     3721 caagaggata tggcgaacga aggtaaccgt gcgatgatgg cgaacatgac cgagaaactg
     3781 ggtccgagcg tggttggtca ggcgggcaag atgatggaaa gcggtatgct gcagcaagcg
     3841 atggcgggtc aaaccccgac catgggccag taatgccagg catcaaatta agcagaaggc
     3901 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
     3961 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4021 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4081 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4141 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4201 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4261 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4321 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4381 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4441 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4501 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4561 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4621 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4681 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4741 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4801 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4861 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4921 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4981 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaa
//
