LOCUS       sequence-369859-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage T3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_T3
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     267..286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     520..537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    552..692
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(618..637)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     778..800
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(956..1834)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1861..2145
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2068..2087
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3807..3834
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3853..3944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3945..4013,4014..4805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4163..4182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4976..5198,1..366)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
       61 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      541 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      601 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
      661 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
      721 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      781 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca
      841 attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga
      901 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
      961 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
     1021 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
     1081 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
     1141 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
     1201 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
     1261 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
     1321 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
     1381 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
     1441 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg
     1501 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
     1561 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
     1621 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
     1681 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
     1741 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
     1801 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
     1861 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
     1921 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
     1981 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
     2041 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
     2101 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
     2161 taacaggagg aattaaccat ggctgattca aaacgtacag gattgggcga agacggtgct
     2221 aaagctacct atgaccgcct aacaaacgac cgtagagcct atgagactcg tgcggagaac
     2281 tgtgcgcaat acaccattcc gtccttgttc ccgaaggagt ccgataacga atctaccgac
     2341 tacacgactc cgtggcaggc tgtaggtgcg cggggtctca acaatctagc ctctaagtta
     2401 atgcttgcgt tattcccgat gcagtcgtgg atgaagctga ccattagcga atatgaggcg
     2461 aagcagcttg ttggagaccc tgatggactc gctaaggtgg acgaaggtct gtcaatggtt
     2521 gagcgcataa tcatgaacta tatcgaatcc aacagttacc gcgtaacact ctttgagtgc
     2581 ctcaagcagt tgatcgtggc tggtaacgcc ctgctttact taccggaacc agaaggtagc
     2641 tacaatccga tgaagctgta ccgattgtct tcttatgttg tccaaagaga cgcatacggc
     2701 aatgtgttac agattgtcac tcgtgaccag atagcctttg gtgctctccc ggaagacgtt
     2761 aggtctgcgg tagagaaatc tggtggtgag aagaagatgg acgaaatggt cgatgtgtac
     2821 acccatgtgt atctcgatga agagtccggc gattacctca agtacgagga agtagaggac
     2881 gttgagattg atggttccga tgccacctat ccgactgacg cgatgcccta cattccggtt
     2941 cgcatggttc gcattgatgg cgagtcttac ggtcgctcct actgtgaaga atacttaggt
     3001 gacttaaggt cgcttgagaa tctccaagag gctatcgtta agatgagtat gattagcgcg
     3061 aaggtcattg gtctggtgaa cccggctggc attacgcagc cccgtagatt aaccaaagct
     3121 cagactggtg acttcgttcc aggccgtcga gaagatattg acttcctgca actggagaag
     3181 caagctgact ttaccgtagc gaaagctgtg agtgaccaga tagaagcacg cttatcgtat
     3241 gcctttatgt tgaactctgc ggtacagcgc acaggcgaac gtgtgaccgc cgaagagatt
     3301 cgatacgttg cgtcagaact ggaagatacg cttggtggcg tctactcgat tctgtctcaa
     3361 gaattgcaat tgcctctggt acgtgtgctc ttgaagcaac tccaagcaac ctcgcagatt
     3421 cctgagctac cgaaagaagc cgttgagcct actatcagta caggtctgga agcaattggt
     3481 cgtggtcaag acctcgataa gctggagcgt tgcatctcag cgtgggcggc tcttgcccct
     3541 atgcagggag acccggacat taatcttgct gtcattaagc tacgcattgc taacgctata
     3601 ggtattgata cttctggtat cctactgacg gatgaacaga agcaagccct tatgatgcag
     3661 gatgcggcac aaacaggcgt cgagaatgct gcggctgctg gtggtgctgg tgttggtgct
     3721 ttggctacct caagtccaga agccatgcaa ggtgctgctg cacaggctgg cctcaacgcc
     3781 acctaatgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     3841 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5101 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5161 actctgtagc accgcctaca tacctcgctc tgctaatc
//
