LOCUS       sequence-369861-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage PhiV1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_PhiV1
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..66
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    81..221
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(147..166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     307..329
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(485..1363)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1390..1674
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1597..1616
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2749..2760
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      3336..3363
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3382..3473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3474..3542,3543..4334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3692..3711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4505..5093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4994..5013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      121 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
      181 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
      241 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      301 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
      361 agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg
      421 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
      481 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
      541 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
      601 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
      661 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
      721 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
      781 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
      841 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
      901 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
      961 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     1021 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     1081 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     1141 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     1201 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     1261 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     1321 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     1381 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
     1441 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
     1501 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
     1561 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
     1621 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
     1681 tttttgggct aacaggagga attaaccatg gcgagcagcc agaagcgtga gggcttcgcg
     1741 gaaaacggtg cgaaggcggt gtatgacgcg ctgaaaaacg atcgtaacag ctacgaaacc
     1801 cgtgcggaaa actgcgcgaa gtataccatc ccgagcctgt ttccgaaaga cagcgataac
     1861 gcgagcaccg actataccac cccgtggcaa gcggttggtg cgcgtggtct gaacaacctg
     1921 gcgagcaagc tgatgctggc gctgttcccg atgcagacct ggatgaagct gaccatcagc
     1981 gagtttgaag cgaaacagct ggttgcgcaa ccggcggagc tggcgaaagt ggaggaaggt
     2041 ctgagcatgg ttgagcgtat cctgatgaac tatattgaaa gcaacagcta ccgtgttacc
     2101 ctgttcgaaa ccctgaagca actggtggtt gcgggcaacg cgctgctgta tatcccggag
     2161 ccggagggtg cgtacaaccc gatgaaactg tatcgtctga gcagctacgt ggttcagcgt
     2221 gacgcgtttg gcaccgtgct gcaaattgtt accctggata agaccgcgta tgcggcgctg
     2281 ccggaggatg tgcgtaacgc gatggatagc ggccaggagc acaaaggtga cgaaatgatc
     2341 gatgtttaca cccacattta tctggacgag gaaagcggcg agtacctgaa gtatgaggaa
     2401 atcgatggcg tggaagttga cggtaccgat gcgagctatc cggtggatgc gtgcccgtac
     2461 atcccggtgc gtatggttcg tattgatggc gaaagctacg gtcgtagcta ttgcgaggaa
     2521 tacctgggcg acctgcgtag cctggagaac ctgcaagaag cgatcgttaa gatgagcatg
     2581 atcagcgcga aagtgattgg cctggttaac ccggcgggta ttacccaagt gcgtcgtctg
     2641 accaaagcgc aaaccggcga cttcgttagc ggtcgtccgg aagatatcag cttcctgcag
     2701 ctggaaaagg cggcggactt tagcgtggcg aaagcggtta gcgagcaaat tgaaggccgt
     2761 ctgagctacg cgtttatgct gaacagcgcg gtgcagcgta ccggcgagcg tgttaccgcg
     2821 gaggaaatcc gttacgtggc gagcgagctg gaagataccc tgggtggcgt ttatagcatt
     2881 ctgagccagg agctgcaact gccgatggtg cgtgttctgc tgaagcagct gcaagcgacc
     2941 aaccagatcc cggaactgcc gaaagaggcg gtggaaccga ccattagcac cggtatggag
     3001 gcgctgggtc gtggtcaaga cctggataag ctggaacgtt gcattgcggc gtggagcgcg
     3061 ctggcgccga tgcagaacga cccggatatc aacattgcga ccatcaaact gcgtattgcg
     3121 aacgcgatcg gcattgatac cagcggtatt ctgaagaccc cggaggaaaa acagcaagag
     3181 atggcggaag cggcgcaagg taccgcgctg gagaacgctg cagctagcgc aggtgcaggt
     3241 gctggtgcac ttgctaccgc aagccctgag aacatggaag cggcggcggc gcaggcgggt
     3301 atggttccga actaatgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     3361 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     3421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3481 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3541 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3601 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3661 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     3721 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3781 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3841 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3901 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3961 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4021 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4081 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4141 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4201 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4261 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4321 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4621 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5161 ctgcgttatc ccctgattct gtggataacc gtattacc
//
