LOCUS       sequence-369862-        5201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_T7
FEATURES             Location/Qualifiers
     source          1..5201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..71
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    86..226
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(152..171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     312..334
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(490..1368)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1395..1679
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1602..1621
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3344..3371
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3390..3481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3482..3550,3551..4342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3700..3719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4513..5101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5002..5021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
       61 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
      121 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      181 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
      241 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      301 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      361 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat
      421 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
      481 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
      541 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
      601 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
      661 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
      721 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
      781 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
      841 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
      901 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
      961 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     1021 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     1081 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     1141 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     1201 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     1261 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     1321 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     1381 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
     1441 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
     1501 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
     1561 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     1621 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     1681 cccgtttttt gggctaacag gaggaattaa ccatggcgga aaagcgtacc ggtctggcgg
     1741 aggatggtgc gaagagcgtg tatgaacgtc tgaaaaacga tcgtgcgccg tacgaaaccc
     1801 gtgcgcagaa ctgcgcgcaa tataccatcc cgagcctgtt cccgaaggac agcgataacg
     1861 cgagcaccga ctatcaaacc ccgtggcaag cggttggtgc gcgtggcctg aacaacctgg
     1921 cgagcaaact gatgctggcg ctgtttccga tgcagacctg gatgcgtctg accatcagcg
     1981 agtacgaagc gaagcaactg ctgagcgacc cggatggtct ggcgaaagtg gatgaaggcc
     2041 tgagcatggt tgagcgtatc atcatgaact acatcgaaag caacagctac cgtgtgaccc
     2101 tgttcgaggc gctgaagcag ctggttgtgg cgggtaacgt tctgctgtat ctgccggagc
     2161 cggaaggcag caactacaac ccgatgaaac tgtatcgtct gagcagctac gtggttcagc
     2221 gtgacgcgtt cggtaacgtg ctgcagatgg ttacccgtga ccaaatcgcg tttggtgcgc
     2281 tgccggaaga tattcgtaag gcggtggagg gccaaggtgg cgaaaagaaa gcggacgaaa
     2341 ccatcgatgt ttacacccac atttatctgg acgaggatag cggtgaatac ctgcgttatg
     2401 aggaagtgga gggcatggaa gttcagggta gcgatggcac ctatccgaaa gaagcgtgcc
     2461 cgtacatccc gattcgtatg gtgcgtctgg acggcgagag ctacggccgt agctatatcg
     2521 aggaatacct gggtgatctg cgtagcctgg agaacctgca agaagcgatt gttaagatga
     2581 gcatgatcag cagcaaagtg attggtctgg ttaacccggc gggtatcacc cagccgcgtc
     2641 gtctgaccaa agcgcaaacc ggtgactttg tgaccggccg tccggaagat atcagcttcc
     2701 tgcagctgga gaagcaagcg gactttaccg tggcgaaagc ggttagcgat gcgattgaag
     2761 cgcgtctgag cttcgcgttt atgctgaaca gcgcggtgca gcgtaccggc gagcgtgtta
     2821 ccgcggagga aatccgttac gtggcgagcg agctggaaga caccctgggt ggcgtttata
     2881 gcattctgag ccaggaactg caactgccgc tggtgcgtgt tctgctgaag cagctgcaag
     2941 cgacccagca aattccggag ctgccgaaag aggcggttga accgaccatc agcaccggcc
     3001 tggaagcgat tggtcgtggc caggacctgg ataagctgga gcgttgcgtt accgcgtggg
     3061 cggcgctggc gccgatgcgt gacgatccgg acatcaacct ggcgatgatc aaactgcgta
     3121 ttgcgaacgc gatcggtatt gataccagcg gcattctgct gaccgaggaa cagaagcagc
     3181 aaaaaatggc gcagcaaagc atgcaaatgg gtatggacaa cggcgcggcg gcgctggcgc
     3241 agggtatggc ggcgcaagcg accgcgagcc cggaggcgat ggcggcggcg gcggatagcg
     3301 ttggtctgca gccgggcatc taatgccagg catcaaatta agcagaaggc catcctgacg
     3361 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     3421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3721 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4201 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4621 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5161 gttctttcct gcgttatccc ctgattctgt ggataaccgt a
//
