LOCUS       sequence-369866-        5150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from Ralstonia phage RsoP1EGY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_RsoP1EGY
FEATURES             Location/Qualifiers
     source          1..5150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(256..1134)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1161..1445
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1368..1387
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3059..3086
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3105..3196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3197..3265,3266..4057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3415..3434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4228..4816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4717..4736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4970..4987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5002..5142
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5068..5087)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
       61 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      121 acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg
      181 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
      241 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
      301 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
      361 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
      421 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
      481 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
      541 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
      601 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
      661 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
      721 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
      781 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
      841 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
      901 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
      961 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     1021 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     1081 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     1141 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     1201 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     1261 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     1321 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     1381 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     1441 tccatacccg ttttttgggc taacaggagg aattaaccat ggagcaggaa aagaaaacct
     1501 gcgcgagcct gtatcaaaaa ctgaccaccg accgtgatcc gttcctgaag cgtgcgtacg
     1561 attgcgcgga actgaccatc ccgagcctgc tgccgcgtga aggtcacaac ggcagcacca
     1621 aactggttac cccgtggcag ggtattggtg cgcgtggtgt taacaacctg gcgagcaaac
     1681 tgctgctgac ccaactgccg ccgggtaccc cgccgttcaa gctgagcatc gacgatttta
     1741 ccctggagga actgaccaag caggaaggta tgcgtgcgaa agtggaggaa ggcctgaaca
     1801 agattgaacg tgcggttcaa accgagatcg aagcgaacta tattcgtgtg gcggcgttcg
     1861 aggcgctgaa gcacctgatc gttagcggta acgcgctgct gtatattccg ccggaaggtg
     1921 gcctgcgtgt gtttcacctg gaccgttacg tggttcgtcg tgatccgatg ggcaacgtgc
     1981 tggacatcat taccaaagag aacgttagcc gtgacgcgct gccggataac ctggtgctgc
     2041 cggacgatac cgaggaaaac caagagccgg cggcgggtac caaggacgtg gaactgtaca
     2101 cccacgttta tcgtcagggt cgtcgttgga aggtttatca agaggtgaaa ggcgttcgta
     2161 tcccgggtac cgaaggcagc tacccgctgg ataaaagccc gtggatcccg gtgcgtttca
     2221 cccagattga cggcgagagc tacggtcgtg gctatgttga ggaatacatt ggtgatctga
     2281 aaagcctgga gggcctgagc caagcgattg ttgaaggtag cgcggcggcg gcgaagattc
     2341 tgtttctggt gaacccgaac ggcaccaccg acatggcgga tgttagcgag gcggaaaacg
     2401 gtgcgttccg tgaaggcgtg gcgaccgaca tcaccgttct gcagctgcaa aaacacaacg
     2461 attttcgtgt ggcgctggag accatgaagg acattaccga acgtctggcg ttcgcgtttc
     2521 tgctgaacag cgcggttcag cgtaacggcg agcgtgtgac cgcggaggaa gttcgttata
     2581 tggcgaacga gctggaaagc gcgctgggtg gcatctacag cattctgagc caggaatttc
     2641 aactgccgct gatcaaacgt attatgtacc agatggagcg tcaaaagcgt ctgccggtgc
     2701 tgccggaagg taccgtgaaa ccgatcattg ttaccggcat cgaagcgctg ggtcgtggca
     2761 acgatctgaa caagctgatc cagttcgttc aaattgcggc gcaggcggcg aacctgccgc
     2821 cggagatcga caaggcggat tttctgaaac gtgcgggtac cgcgctgggc attgacatga
     2881 aaggtctggt gctgccgccg gaagtggttg cgcagaacaa ccagcaagcg atgatgatgc
     2941 aaatgatgca gcaaggcgtt aacccggcga tcacccaggc gggtcaactg atgaagcagg
     3001 gcatgcaaaa cgcggcgcag ccggcgggtg gccaataatg ccaggcatca aattaagcag
     3061 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     3121 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3181 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3241 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3301 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3361 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3421 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3481 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3541 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3601 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3661 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3721 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3781 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3841 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3901 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3961 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4021 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4981 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5041 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5101 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
//
