LOCUS       sequence-369868-        5153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from Sphingopyxis flava R11H prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_prophage_Sphingopyxis_flava
FEATURES             Location/Qualifiers
     source          1..5153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(34..912)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        939..1223
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1146..1165
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      2840..2867
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2886..2977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2978..3046,3047..3838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3196..3215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4009..4597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4498..4517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4751..4768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4783..4923
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4849..4868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5009..5031
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
       61 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
      121 tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
      181 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
      241 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
      301 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
      361 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
      421 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
      481 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
      541 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg
      601 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
      661 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
      721 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
      781 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
      841 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
      901 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
      961 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac
     1021 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
     1081 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
     1141 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
     1201 gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa ttaaccatgg
     1261 cgcagaaggc ggatggtctg cgtagcggcg aggcgaagac ccgttatgaa cagctgaaac
     1321 gtgcgcgtga gaacgttctg acccaagcgc gtgaagcggc gcgtctgacc attccgggtc
     1381 tggttccggc ggagggtgcg accgacccgc accaagtgag cgaacaaccg tacaccgcga
     1441 acggtgcgcg tttcgtgaac aacctgagcg cgaaactgct gctggcgctg tttccgccgg
     1501 agcgtccgtt ctttcgtctg gatattggtc cggatgttgc ggaggaaatg ggtgcggcgt
     1561 atggcgaggc gcaggaaaaa ctggcggata ttagccgtcg tgcgatggtg ctggcggagg
     1621 acagcgcgag ccgtaccatc tggatggaaa ccctgcgtca cctggtggtt gcgggcaacg
     1681 cgctggttta ccagccggac gatggtaccc aaatgcgtat gtggcgtatt gatcagttcg
     1741 tggttgtgcg tggccgtgac ggtctgctgc aagaggcggt gatcaacgat aagatttatc
     1801 cgagcgaact gagcgaggaa gttcgtgcgg cgtgcaacgt gaagtacgac ccgagcaaac
     1861 cggaccagaa cgatccggtt gacctgtaca cccacatcta tctgaacggt gatcgtatgg
     1921 agcactatga ggaaatcaac ggcattgagg ttccggaaag ccgtggtagc gtggaacgta
     1981 aaagcggtgg ctggcaagcg ctgcgttggc aagcggttcc gggtagcgac tatggtcgtg
     2041 cgtatgtgac cgagtacgcg ggtgatttcc tgagcctgga agacgcgcac agcagcatcc
     2101 tgaaattcgc gattgaggcg gcgcgtatcc tgcgttttgt tgatccgaac gcgggtattg
     2161 acgttgagga agtgacccgt gcggaaagcg gcgattttct gaccggtatg atcgaccgtg
     2221 ttcagaccct gcaactggat aaaaaccagg acttccaaat tgtgtggaac caggcgcaaa
     2281 gcatcgagcg tcgtctgagc caggcgtttc tgattgcgag caacgcgatc cgtgatgcgg
     2341 aacgtgtgac cgcggaggaa atccgtgcga ttgcgcaaga gctggaagac agcctgggtg
     2401 gcacctacac cgttctgagc gcggaagtgc agcgtccgta tgcgagccgt ctgctgtaca
     2461 ttctgagcaa gcaaaagaaa gcgccgaaac tgccggaaag cgttagcgtt gtgatcgtga
     2521 ccggctttaa cgcgctgggt cagaaccatg agagcgcggc gctgaagggt tggctgatgg
     2581 atgttcgtga cgcgctgggt gaagcgtgga tcagccagaa cctggatggc caggcggttg
     2641 cgctgcgtct gggtgtgggt cgtggtgttg tggacgttag caagctgatt aaagcggcgg
     2701 aggatgtggc ggaagatgcg caagcgggtg cgctgaccca agcgggtatc agcgcggcgc
     2761 cgcagattgc gaaaggtgtg attgatgcgg cgagcgcggc gaaccaagaa ggcacctaat
     2821 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     2881 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2941 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3001 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3061 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3121 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3181 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     3241 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3301 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3361 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3421 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3481 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3541 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3601 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3661 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3721 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3781 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3841 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3901 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3961 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4141 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4621 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4681 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4741 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     4801 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     4861 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     4921 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     4981 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5041 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca
     5101 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctc
//
