LOCUS       sequence-369869-        3666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A13_STOP
FEATURES             Location/Qualifiers
     source          1..3666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1223..1322)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1339..1358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1340..1358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1363..1379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1363..1379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1481..1500)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1492..2301
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1559..1578)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2448..3036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2937..2956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3190..3207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3366..3393)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3485..3571)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3620..3642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3634..3651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3635..3651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc aggctgccca
      121 tgtcctggat gcggaatggc aggaccaagg ctgctgttcc tgaccgccct ggccctggag
      181 ctgctggaga gggcaggagg atcccagccc gccctgcggt ctagaggaac cgcaacagca
      241 tgcaggctgg acaataagga gagcgagtcc tggggcgccc tgctgtccgg agagagactg
      301 gatacctgga tctgttccct gctgggctct ctgatggtgg gcctgtctgg cgtgtttccc
      361 ctgctggtca tccctctgga gatgggaaca atgctgcgga gcgaggcagg agcatggaga
      421 ctgaagcagc tgctgtcctt cgccctggga ggactgctgg gcaacgtgtt tctgcacctg
      481 ctgccagagg catgggcata cacctgctct gccagccctg gaggagaggg acagagcctg
      541 cagcagcagc agcagctggg actgtgggtc atcgcaggca tcctgacatt cctggccctg
      601 gagaagatgt ttctggacag caaggaggag ggcacctccc aggcaccaaa taaggatccc
      661 acagcagcag cagccgccct gaacggagga cactgtctgg cacagcctgc agcagagcca
      721 ggactgggcg ccgtggtgag gagcatcaag gtgtccggct atctgaacct gctggccaat
      781 accatcgaca acttcacaca cggactggca gtggcagcat cttttctggt gagcaagaag
      841 atcggcctgc tgaccacaat ggccatcctg ctgcacgaga tccctcacga agtgggcgac
      901 ttcgcaatcc tgctgagggc aggctttgat cgctggtctg ccgcaaagct gcagctgagc
      961 accgccctgg gcggcctgct gggagcaggc ttcgccatct gcacacagtc cccaaaggga
     1021 gtggtgggat gttctccagc agcagaggag accgcagcat gggtgctgcc attcacatcc
     1081 ggcggctttc tgtacatcgc cctggtgaat gtgctgcctg acctgctgga ggaggaggat
     1141 ccatggcggt ctctgcagca gctgctgctg ctgtgcgcag gaatcgtggt catggtgctg
     1201 ttcagcctgt ttgtggatta agacccagct ttcttgtaca aagttggcat tataagaaag
     1261 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1321 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1381 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1441 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1501 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1561 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1621 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1681 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1741 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1801 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1861 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1921 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1981 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2041 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2101 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2161 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2221 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2281 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2341 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2581 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2821 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3001 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3061 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3301 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3361 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3421 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3481 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3541 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3601 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3661 gggccc
//
