LOCUS       sequence-36987-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
  TITLE     Dynamic analysis of cytosolic glucose and ATP levels in yeast using 
            optical sensors.
  JOURNAL   Biochem J. 2010 Dec 1. 432(2):399-406.
  PUBMED    20854260
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2010 Dec 1. 432(2):399-406."
COMMENT     Sequence Label: pDR-GW AT1.03R122K/R126K
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     455..476
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(579..600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(840..859)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atctcggcat cctccccggt cacattccca caaaggcccc actgaagatc ggagctgtgc
       61 ggctgaagaa ggacggccaa accgagatgg tcgcagtctc aggcggcact gttgaagtgc
      121 ggcctgacca cgttaccatt aatgctcaag ccgctgaaac agccgaagga atcgacaaag
      181 agagagcaga agccgcaaga cagagggccc aggagcggct gaactctcaa tccgatgaca
      241 ccgatattcg ccgggccgag ctggcactgc agaaggccct gaacaagctg gacgtggctg
      301 ggaaggcaaa cgaattcatg gacggcggcg tgcagctcgc cgaccactac cagcagaaca
      361 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
      421 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      481 ccgccgggat cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga
      541 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      601 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      661 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      721 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      781 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgtaccatc ttcttcaagg
      841 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      901 gcatcgagct gaagggcatc gact
//
