LOCUS       sequence-369870-        4008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC30A10_STOP
FEATURES             Location/Qualifiers
     source          1..4008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1565..1664)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1681..1700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1682..1700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1705..1721)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1705..1721)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1823..1842)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1834..2643
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1901..1920)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2790..3378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3279..3298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3532..3549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3708..3735)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3827..3913)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3962..3984
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3976..3993
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3977..3993
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg ccgctactcc
      121 ggcaagacct gcaggctgct gttcatgctg gtgctgacag tggccttctt tgtggccgag
      181 ctggtgtccg gctatctggg caactctatc gccctgctgt ccgactcttt taatatgctg
      241 tccgatctga tctctctgtg cgtgggactg agcgccggat acatcgcacg gagacctacc
      301 agaggcttct ccgccacata cggctatgca agggcagagg tggtgggcgc cctgagcaac
      361 gccgtgtttc tgaccgccct gtgcttcaca atctttgtgg aggccgtgct gaggctggca
      421 agacctgaga ggatcgacga tccagagctg gtgctgatcg tgggcgtgct gggcctgctg
      481 gtgaatgtgg tgggcctgct gatcttccag gactgcgcag catggtttgc atgctgtctg
      541 aggggcagat ccaggcgcct gcagcagagg cagcagctgg ccgagggatg cgtgccagga
      601 gccttcggcg gacctcaggg cgccgaggac ccaaggagag cagcagatcc aaccgcacca
      661 ggaagcgatt ccgccgtgac cctgaggggc acatctgtgg agaggaagcg cgagaaggga
      721 gcaaccgtgt tcgcaaacgt ggcaggcgac tcctttaaca cacagaatga gcctgaggat
      781 atgatgaaga aggagaagaa gtctgaggcc ctgaatatca gaggcgtgct gctgcacgtg
      841 atgggcgacg cactgggatc cgtggtggtg gtcatcacag ccatcatctt ctacgtgctg
      901 cccctgaagt ctgaggaccc ttgcaactgg cagtgttata tcgatccaag cctgaccgtg
      961 ctgatggtca tcatcatcct gagctccgcc tttcctctga tcaaggagac agccgccatc
     1021 ctgctgcaga tggtgccaaa gggcgtgaat atggaggagc tgatgtctaa gctgagcgcc
     1081 gtgccaggca tctctagcgt gcacgaggtg cacatctggg agctggtgag cggcaagatc
     1141 atcgccaccc tgcacatcaa gtaccccaag gaccgcggct atcaggatgc ctccaccaag
     1201 atccgggaga tcttccacca cgccggcatc cacaacgtga caatccagtt tgagaatgtg
     1261 gacctgaagg agccactgga gcagaaggat ctgctgctgc tgtgcaacag cccctgtatc
     1321 tccaagggat gcgcaaagca gctgtgctgt ccacctggcg ccctgccact ggcacacgtg
     1381 aacggctgtg ccgagcacaa tggcggccct tctctggaca cctacggatc tgatggactg
     1441 agcaggaggg acgcaagaga ggtggcaatc gaggtgtccc tggactcttg cctgagcgat
     1501 cacggccaga gcctgaacaa gacccaggag gatcagtgtt atgtgaatag gacacacttt
     1561 taagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1621 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1681 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1741 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1801 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1861 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1921 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1981 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2041 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2101 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2161 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2221 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2281 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2341 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2401 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2461 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2521 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2581 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2641 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2701 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2761 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2821 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2881 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2941 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3001 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3061 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3121 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3181 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3241 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3301 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3361 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3421 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3481 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3541 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3601 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3661 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3721 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3781 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3841 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3901 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3961 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
