LOCUS       sequence-369871-        8627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon optimized KpAvs4 with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: pCDF-KpAvs4
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        786..877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             878..1669
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(1840..1887)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1883..1901
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1933..1950)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    7103..7127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7127..7146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7128..7146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        7192..7269
                     /gene="lacI"
                     /label=lacI promoter
     CDS             7270..8352
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7289..7308)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cacctgggga ggtggtttga tcgaaggtta agcagttccc caactgactt aaccttcgaa
       61 aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg
      121 tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt
      181 tggcgcacac ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag
      241 ttaccaatgg ctgctgccag tggcgtttta ccgtgctttt ccgggttgga ctcaagtgaa
      301 cagttaccgg ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc
      361 ttggagcgaa cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca
      421 cacttcccgt aagggagaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg
      481 agagcgcaag agggagcgac ccgccggaaa cggtggggat ctttaagtcc tgtcgggttt
      541 cgcccgtact gtcagattca tggttgagcc tcacggctcc cacagatgca ccggaaaagc
      601 gtctgtttat gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg
      661 ccctgctgtt ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt
      721 tgtatgtgtc cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagct
      781 agctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agggaagcgg tgatcgccga
      901 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      961 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1021 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1081 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1141 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1201 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1261 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1321 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1381 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     1441 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1501 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1561 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1621 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat
     1681 tcgttcaagc cgaggggccg caagatccgg ccacgatgac ccggtcgtcg gttcagggca
     1741 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
     1801 cagtgtgacc gtgtgcttct caaatgcctg aggtttcagc aaaaaacccc tcaagacccg
     1861 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag cctaggttaa
     1921 ttagccgctg ctgtggtgat gatggtgatg gcttccgccg gtgaaaacac gacggctacg
     1981 gatcaggttc tcacgcagat agtacgcacg cgcgctgccg gtttccacaa taccgctcag
     2041 cagggtcatg cagctgttgt acattttacg gctcaggatg ccacggttct caatctgcag
     2101 ataacgacca atcgccattt ccaggtagaa cgcggtgttg atttgtttat caatcagctt
     2161 gctgttcatg atcagtttct ccgccaggat gtgaatgcct tcgtcaaaga agatgtggtg
     2221 gaagtgataa atcaggctgc tcagcgccat aaaaacgtgg ctgttgttgc cgctgatctt
     2281 cgcaaagtcc agaatgtgca cgctaccgta cgcgatgtca cgcgcttcgt tctcgtggcc
     2341 ctgccaacgg gtatccgcat acagcatacc acgcagcagc tggttcaggt cgctttggat
     2401 accgctgtac tgctcgttaa catctttcag cgcaatcttg tgcgcctccg gcgccagcat
     2461 cagccagaat tcccacaggc tctccaggtc ttccgctttc tccatcgcga tgtgaaacag
     2521 cagcttggtc atataaacaa tacgcggcgc ggtgctgcaa ccttccagca gcagggtttt
     2581 aaacggcacg aaaccggtct tacgggtgtg gatgaagtgc tccgcaaagc agttggtgat
     2641 ctgcttacga atgtcgtgtt ggatggtttc gtcatcgtgg ttgcttttgt gacgcttata
     2701 ttcctcatca aaggtgaagt gaacgatttt cttgtacagg ttaatcagca gcgggttgtc
     2761 cacgcagaac gggatcatca gaatcggcag gtggatgtgc cagctgctgt gggttttcag
     2821 gctgatgttc tcaatcagcg gcggggtggt gttaaagaat tcctcacgga agctggtgat
     2881 caggtagctc cagtgcgcct tcgcctgcgc cagctcggta tcgctataca ggtaaaaacg
     2941 ttggctgttg atgttttctt tcttaaaacg cgcgaacatg gtcgcaccat aaatgcactg
     3001 gaacgccagg gtcgggtggg tggtccacag aaactcacgc acgcctttcg ccgcgtactc
     3061 gccgatgtca atgttttcgt gggtcagcgc gatcgccagg gtgaacttca gttcgttcag
     3121 ttcctcgtcg tttttaacat aacggaacag cttcggcagc acgaacgcgc acgcaccgct
     3181 accatagctg tcggttttat cgtgcaggct caggccgttg atgcagtccg cgttcatgat
     3241 aatgcactcg ataatgattt tcttgcacca gttcttctcg tcctcgctca ggttttcgaa
     3301 caggtcacgg gtgcaaaccg ccgcggtctt ggtgatggta ccaataacca tcaccgggtt
     3361 gtccacctcg ttgttctgaa atttatcgaa caggtacttc gccgcggtca gcgcttcggt
     3421 gtggctgtta aagtaggtgt tgttgatgtt ttcctcgaag cttttacggc tccacagaaa
     3481 cagcttgttg atcaggctgt tttgggtgtg ggtcgcgctg tgctcgtcgt gaatttgctg
     3541 cagatcttcc ggcagctccg ggttgctctg cgcaatgata cggctgtttt ccttgtccgc
     3601 ggtgaagctc caggtacggg tatccgcacg gtgaaccaga aaacgcaggt tctcgtcgtt
     3661 gctttccagg ctcagcgcga tcaggctgtc cacaattttc agggtatccg ctttgttctt
     3721 gccgctcagc tgcagacggg tcagcaggct ttccagggtc tccttacgcc acggacgcag
     3781 cgccgcaata cgacgctcct caacataggt gtcgttcata atatcgttgt ggaacatgtt
     3841 gctaaaccag ttctgctcgc tgccaccacg ttcgttcacc agacgttgga tgtccaggtt
     3901 gtagaactcc gggcaacgca ggatcggaaa cgccgctttg ctaatcttgt ccgggaaacc
     3961 ggtcgcaacg ctcgccagca cgctggtcgg cataacgctg ttgctgctca gcaggatgta
     4021 gttaaacagc cactcaatgg tgtcgttctt ctccaggctg tccaggctat cgatcagcca
     4081 gttctccagc gccatcagcg cgcattgcag aatatacggc agggtgctgt ggccacggta
     4141 acctttccac aggtgcgggc tgctgaactg ggtaaccacg gtgccgtcgt tcagggtcag
     4201 ctcaacgttc ttcaccaggg tttcatcgct catgaaaccg ctgctacggg tcgcaaattc
     4261 gctggtgcta tatttcttcg cggttttgtt gcacaggtca atgataaaat ccagcgcctt
     4321 acgcgggtgg aagtgcagca ggtatttaaa cgggcccttc gcaccgctcg ccggaaagaa
     4381 gtcgctctca cggttcaggc cgaaatactg atccacaccg gtggtttgtt tgtaaaagat
     4441 gctgtcatcg tcttcaatgt tgtcttgcag ccactcgtga cgcgccagac gttcgatgaa
     4501 atccggacgg tacttgcaca gaatcggaac gttcagaccg atcaggctct ggttaatcag
     4561 atactccacg tagtgcagac gacggtggct ggtgtccggg atgaaaacct ctttatccat
     4621 cagcaggtca aattcatcca cgatggtcgg gctcagcttc agcagcaggc taataacttt
     4681 ctcacgctta tattcgtcct cacgcgcgta gctatctttc agcggttcca gcagccagat
     4741 cgccagcagg cccactttac ggctaatgtc cggatagtta tcgctgatgt tcagaatgct
     4801 aacccagctg ttcagcatct caatgatgtg gatgctggtg ctgttcacca gctcgttctg
     4861 gttttcaaag atgaagttaa tcagcgcgtc ccagcagata ccatacggtt gcaggtacag
     4921 ggttttgcta atttcctgct cctctttttc cagctcttgg ttgtacaggg tcttcggttg
     4981 ctggcaggtc atacgcagga tgaacagaac acgaatcagc agcaggtttt tgttgctcag
     5041 cagctgggta cggaacatct tcagaaagat gttcaccgcg tcgctttgca ggatcgcggt
     5101 aatggtctca tctttccaat agctcgcaat gctcgcgtcg gtcagaacct tgtagatgaa
     5161 atcgttgatt tccttgttct ccttgatgtt acggctcagc cacagacgat acgcacggtt
     5221 gatgctcggc tcgtcgccaa tctgttcaaa gaatttgttc ggaaccagga agttttccac
     5281 gtactcgttg tcgataaaaa tatccagcgc ccagtcctcc agcacatcgt ggctcgggct
     5341 gatcaggttc agacgttcgt gacgttgaat caggttgtct tcctccagct tcgcaatgat
     5401 ttccggatcg aacgcatcgt ccaggatacc aaccaccatt ttcttcgcac gctgtttcgc
     5461 gatgtcaata aaggtacgtt tacgcttcgc cggcatgcca ccacgacggt cggtctcttt
     5521 cgcgataacg ctgctccaaa tgatttcacg gaaatcacgt tcggtgccct cggtgtgaaa
     5581 ttgcgcaccg tttttcagcg cacgaaccgc cacgtcgata aagaacggca ccttcagcag
     5641 atcggtcaga cgtttgttct caatcagctt ttccagctcc ggaattttgt ggcagatttc
     5701 gctcagttgc tggtactcca gcgccttgat ttcaacgttg caataatcaa taccgctcgg
     5761 ctgcaggtag ttgaagatca gttgctgggt cgcatagtca cggccggtcg caatcacggt
     5821 ccaaccgttc tggtttttga tgaagtgcag cagatcaata aacgcgtttt ggtaggtcag
     5881 ctccagaacc ttttccacgc tctcaatgat caggattttt tccttcagca ggctgaactc
     5941 gcccgcaatt tccgccaggc tgcagctcat acccagcgcg gtaaaaacgt cgttcaggtg
     6001 gctcttgtcc agatcctcgg tacgcagata aaagaacggc acgtctttgt acttggtgct
     6061 gatgaaatct ttaacaatgc cgctcttgcc cacaccacgg ataccggtaa caaacacgaa
     6121 acggctctcc tggcatttct tttcaatctc cgccagttgt tcgttacggt tgatgtgaat
     6181 gccgcaaata cggttgctga tgccggtata aatgtaggta ccacgttcct gcagtttacg
     6241 gatgtcgtcc ttgatgtttt tcttgtgaac cttgttgaac agggtcttca gatcgtgcgg
     6301 gatgttttcc tcgctcagaa tacccgcgtt ttggttaaag ttctgaatgg tggtaatgat
     6361 tttgctcagc accagcggcg gcgcctcatc gctgttgttt tggatctggc tgcacagcag
     6421 gctcgcaacc acgctgaagc tgctgtccag atcgtagcta atcaggtgga agctcttcag
     6481 aaagctccac aggaaggtat cgtcgatttc gctaatgttg ctcgcgtgac gcaggtgcag
     6541 acggaagttc tcgattttct tcagtttcgc ttcgctgcta aagcccttgg tctgaacttt
     6601 cttcatgaaa tcctccgcgc tcagcgcaaa tttcgcccat tccaggatcg gcagggtgtt
     6661 gtgaatgtcg gtcttttgca gcggaccggt gatcagaaca atcacgtcgt gatccatttt
     6721 gaagttgtcg ctcttaaaat cctgccacgc gctcgcgatc acttccgcga acacgctatc
     6781 cgggctctcg ctcagggtga tttcgtgttt aatctgcgca aacagcttgc cctcttgacc
     6841 gtcgctatct tccgcaacga tcacgaaatc gtcggtgttg ctgccgtcgt atttgttttg
     6901 aaaacgaatc tgcttcgcac gcatgctttg gctcaggcac ggcacgctgc taccggtcag
     6961 gatgctcagc gcaaacgccg cttgaacacg ggtctcaaag ttcacgccac cgccaccggt
     7021 gctgaacggg ttgctttgtt tcttcaggtt ttcgttcatg gtatatctcc ttattaaagt
     7081 taaacaaaat tatttctaca ggggaattgt tatccgctca caattcccct atagtgagtc
     7141 gtattaattt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc
     7201 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     7261 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     7321 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     7381 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     7441 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     7501 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     7561 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     7621 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     7681 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     7741 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     7801 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     7861 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     7921 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     7981 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     8041 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa
     8101 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     8161 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     8221 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     8281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8341 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc
     8401 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     8461 gactaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
     8521 gagataaatt gcactgaaat ctagagcggt tcagtagaaa agatcaaagg atcttcttga
     8581 gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaac
//
