LOCUS       sequence-369872-        3256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a human siRNA-expressing plasmid targeting HBV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  TITLE     Zeng Yan Lab Plasmids 2022
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSuper-si-HBV
FEATURES             Location/Qualifiers
     source          1..3256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     291..312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     576..598
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     590..607
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     591..607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        617..635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     739..755
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     740..756
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(771..985)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(831..850)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(1005..1021)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1005..1021)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1058..1078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1058..1076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1097..1113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1097..1113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1110..1132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1121..1137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1145..1151,1152..1169,1170..1175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1190..1211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1328..1345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1499..2087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1579..1598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2258..3049,3050..3118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2881..2900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3119..3223)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3250..3256,1..450)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
       61 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      121 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      181 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      241 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      301 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
      361 tttgccgatt tcggcctatt ggttaaaaaa atgagctgat ttaacaaaaa tttaacgcga
      421 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
      481 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      541 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      601 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccc gggatctaga
      661 aaaaaactcg aggcgactga gagtccaaga gtcctcttgt gaagccacag aaagaggact
      721 cttggattct tagctgcctc gaggtcgacg gtatcgataa gcttagatct gtggtctcat
      781 acagaactta taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa
      841 cacatagcga catgcaaata ttgcagggcg ccactcccct gtccctcaca gccatcttcc
      901 tgccagggcg cacgcgcgct gggtgttccc gcctagtgac actgggcccg cgattccttg
      961 gagcgggttg atgacgtcag cgttcgaatt cctgcagccc gggggatcca ctagttctag
     1021 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     1081 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1921 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3241 tgccacctga cgcgcc
//
