LOCUS       sequence-369874-        4626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A12_STOP
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2183..2282)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2299..2318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2300..2318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2323..2339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2323..2339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2441..2460)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2452..3261
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2519..2538)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3408..3996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3897..3916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4150..4167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4326..4353)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4445..4531)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4580..4602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4594..4611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4595..4611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgtg cttccgcacc
      121 aagctgagcg tgtcctgggt gcctctgttc ctgctgctgt ctcgggtgtt tagcacagag
      181 accgacaagc catctgccca ggattctcgc agcaggggaa gctccggaca gcctgcagac
      241 ctgctgcagg tgctgagcgc cggcgatcac ccacctcaca accactcccg ctctctgatc
      301 aagaccctgc tggagaagac aggctgccct cggagaagga acggcatgca gggcgactgc
      361 aacctgtgct tcgagccaga tgcactgctg ctgatcgcag gaggcaattt tgaggaccag
      421 ctgagggagg aggtggtgca gcgggtgtcc ctgctgctgc tgtactatat catccaccag
      481 gaggagatct gttctagcaa gctgaacatg tctaataagg agtacaagtt ctatctgcac
      541 tctctgctga gcctgagaca ggacgaggat tcctcttttc tgagccagaa cgagaccgag
      601 gacatcctgg ccttcaccag gcagtacttt gatacaagcc agtcccagtg catggagaca
      661 aagaccctgc agaagaagag cggaatcgtg agctccgagg gagcaaacga gtccaccctg
      721 ccacagctgg ccgccatgat catcacactg tccctgcagg gcgtgtgcct gggacaggga
      781 aatctgcctt ctccagacta cttcaccgag tatatctttt ctagcctgaa cagaacaaat
      841 accctgaggc tgagcgagct ggatcagctg ctgaacacac tgtggaccag atccacatgc
      901 atcaagaatg agaagatcca ccagttccag aggaagcaga acaatatcat cacccacgac
      961 caggattaca gcaatttttc ctctagcatg gagaaggagt ctgaggacgg ccccgtgagc
     1021 tgggatcaga catgtttctc cgccagacag ctggtggaga tctttctgca gaagggcctg
     1081 tccctgatct ctaaggagga cttcaagcag atgagcccag gcatcatcca gcagctgctg
     1141 tcttgcagct gtcacctgcc caaggatcag caggccaagc tgccacccac cacactggag
     1201 aagtacggct attctaccgt ggccgtgacc ctgctgacac tgggcagcat gctgggcaca
     1261 gccctggtgc tgttccactc ctgcgaggag aactataggc tgatcctgca gctgtttgtg
     1321 ggactggcag tgggcaccct gagcggcgac gcactgctgc acctgatccc tcaggtgctg
     1381 ggactgcaca agcaggaggc accagagttc ggccactttc acgagtccaa gggccacatc
     1441 tggaagctga tgggcctgat cggcggcatc cacggcttct ttctgatcga gaagtgtttc
     1501 atcctgctgg tgtcccccaa cgataagcag ggcctgtctc tggtgaatgg acacgtggga
     1561 cactcccacc acctggccct gaatagcgag ctgtccgacc aggcaggaag gggcaagtcc
     1621 gcctctacca tccagctgaa gtcccccgag gattctcagg ccgccgagat gcctatcggc
     1681 agcatgacag cctccaaccg gaagtgcaag gccatctctc tgctggccat catgatcctg
     1741 gtgggcgaca gcctgcacaa tttcgcagat ggactggcaa tcggagcagc cttttcctct
     1801 agctccgaga gcggcgtgac cacaaccatc gccatcctgt gccacgagat cccccacgag
     1861 atgggcgact ttgccgtgct gctgtctagc ggcctgtcca tgaagaccgc catcctgatg
     1921 aacttcatct cctctctgac agcctttatg ggcctgtaca tcggactgag cgtgtccgcc
     1981 gacccttgcg tgcaggattg gatcttcaca gtgaccgccg gcatgtttct gtatctgagc
     2041 ctggtggaga tgctgccaga gatgacccac gtgcagacac agcggccctg gatgatgttc
     2101 ctgctgcaga actttggcct gatcctggga tggctgtccc tgctgctgct ggccatctat
     2161 gagcagaata tcaagatcta agacccagct ttcttgtaca aagttggcat tataagaaag
     2221 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     2281 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2341 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2401 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2461 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2521 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2581 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2641 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2701 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2761 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2821 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2881 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2941 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     3001 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     3061 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     3121 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     3181 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     3241 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     3301 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3361 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3421 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3481 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3541 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3601 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3661 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3721 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3781 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3841 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3901 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3961 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4021 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4081 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4141 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     4201 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     4321 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4381 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     4441 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4501 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4561 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4621 gggccc
//
