LOCUS       sequence-369876-        5768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage P22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_P22
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     411..430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     664..681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    696..836
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(762..781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     922..944
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1100..1978)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2005..2289
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2212..2231
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      4521..4548
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4567..4658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4659..4727,4728..5519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4877..4896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5690..5768,1..510)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
       61 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      601 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      661 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
      721 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
      781 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
      841 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      901 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      961 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg
     1021 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
     1081 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
     1141 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     1201 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     1261 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     1321 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     1381 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     1441 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     1501 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     1561 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     1621 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     1681 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     1741 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     1801 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     1861 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     1921 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     1981 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     2041 gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa
     2101 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     2161 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     2221 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     2281 tttctccata cccgtttttt gggctaacag gaggaattaa ccatggcgga caacgaaaac
     2341 cgtctggaga gcatcctgag ccgtttcgat gcggattgga ccgcgagcga tgaagcgcgt
     2401 cgtgaggcga aaaacgacct gttcttcagc cgtgtgagcc agtgggacga ttggctgagc
     2461 caatacacca ccctgcagta tcgtggtcaa tttgacgtgg ttcgtccggt ggttcgtaaa
     2521 ctggttagcg agatgcgtca gaacccgatt gacgttctgt accgtccgaa agatggtgcg
     2581 cgtccggatg cggcggatgt tctgatgggc atgtatcgta ccgatatgcg tcacaacacc
     2641 gcgaagatcg cggtgaacat tgcggttcgt gaacaaattg aagcgggtgt gggtgcgtgg
     2701 cgtctggtta ccgactacga ggatcagagc ccgaccagca acaaccaagt gattcgtcgt
     2761 gaaccgatcc acagcgcgtg cagccacgtt atttgggaca gcaacagcaa actgatggac
     2821 aagagcgatg cgcgtcactg caccgtgatc cacagcatga gccagaacgg ttgggaagat
     2881 ttcgcggaga aatacgacct ggatgcggac gatattccga gcttccaaaa cccgaacgac
     2941 tgggtttttc cgtggctgac ccaggatacc atccaaattg cggagttcta tgaagtggtt
     3001 gagaagaaag aaaccgcgtt tatctaccag gacccggtga ccggcgagcc ggttagctat
     3061 ttcaaacgtg acatcaagga tgtgattgac gatctggcgg atagcggctt tatcaagatt
     3121 gcggaacgtc agattaaacg tcgtcgtgtg tacaagagca tcattacctg caccgcggtt
     3181 ctgaaagaca agcaactgat cgcgggcgag cacatcccga ttgtgccggt tttcggtgaa
     3241 tggggctttg tggaggacaa agaagtttat gagggtgtgg ttcgtctgac caaggatggc
     3301 cagcgtctgc gtaacatgat tatgagcttc aacgcggaca tcgtggcgcg taccccgaag
     3361 aaaaagccgt tcttttggcc ggaacaaatc gcgggttttg agcacatgta cgatggcaac
     3421 gacgattatc cgtactatct gctgaaccgt accgacgaga acagcggtga tctgccgacc
     3481 cagccgctgg cgtactatga aaacccggaa gtgccgcaag cgaacgcgta catgctggag
     3541 gcggcgacca gcgcggtgaa agaagttgcg accctgggcg tggacaccga agcggttaac
     3601 ggtggccagg tggcgttcga taccgttaac caactgaaca tgcgtgcgga cctggagacc
     3661 tatgtgtttc aggataacct ggcgaccgcg atgcgtcgtg acggtgaaat ctaccaaagc
     3721 attgttaacg acatctatga tgtgccgcgt aacgttacca tcaccctgga agacggtagc
     3781 gagaaagatg tgcagctgat ggcggaagtg gttgatctgg cgaccggcga aaagcaagtg
     3841 ctgaacgaca ttcgtggtcg ttacgagtgc tataccgatg ttggcccgag cttccagagc
     3901 atgaaacagc aaaaccgtgc ggaaatcctg gagctgctgg gcaagacccc gcaaggtacc
     3961 ccggagtacc agctgctgct gctgcaatat tttaccctgc tggacggtaa aggcgttgaa
     4021 atgatgcgtg attacgcgaa caagcagctg attcaaatgg gtgtgaaaaa gccggagacc
     4081 ccggaggaac agcaatggct ggttgaagcg cagcaagcga agcaaggcca gcaagacccg
     4141 gcgatggtgc aggcgcaagg tgttctgctg cagggccaag cggagctggc gaaagcgcag
     4201 aaccaaaccc tgagcctgca gatcgacgcg gcgaaggtgg aggcgcagaa ccaactgaac
     4261 gcggcgcgta tcgcggaaat tttcaacaac atggatctga gcaaacagag cgaattccgt
     4321 gagtttctga agaccgttgc gagctttcag caagaccgta gcgaagatgc gcgtgcgaac
     4381 gcggagctgc tgctgaaagg tgacgaacag acccacaagc aacgtatgga tatcgcgaac
     4441 attctgcaga gccaacgtca gaaccaaccg agcggcagcg ttgcggaaac cccgcagtaa
     4501 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
     4561 aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4621 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4681 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4801 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4861 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     4921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4981 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5041 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5221 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5281 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5341 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5761 gtttgccg
//
