LOCUS       sequence-369878-        3579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC39A11_STOP
FEATURES             Location/Qualifiers
     source          1..3579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1136..1235)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1252..1271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1253..1271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1276..1292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1276..1292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1394..1413)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1405..2214
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1472..1491)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2361..2949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2850..2869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3103..3120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3279..3306)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3398..3484)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3533..3555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3547..3564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3548..3564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgct gcagggccac
      121 agctccgtgt tccaggccct gctgggcacc ttctttacat ggggaatgac cgcagcagga
      181 gccgccctgg tgttcgtgtt ttctagcgga cagaggagaa tcctggacgg aagcctggga
      241 tttgcagcag gcgtgatgct ggcagcatct tactggagcc tgctggcacc agcagtggag
      301 atggccacct cctctggagg attcggagcc tttgccttct ttcctgtggc agtgggcttc
      361 acactgggag cagccttcgt gtacctggca gacctgctga tgccacacct gggagcagca
      421 gaggatccac agaccacact ggccctgaac ttcggcagca cactgatgaa gaagaagagc
      481 gacccagagg gacccgccct gctgtttcca gagtccgagc tgtctatcag gatcggaaga
      541 gcaggactgc tgagcgataa gtccgagaat ggagaggcat accagcggaa gaaggcagca
      601 gcaaccggac tgcctgaggg accagccgtg cccgtgccta gccggggcaa cctggcccag
      661 ccaggcggca gctcctggag gcgcatcgcc ctgctgatcc tggccatcac aatccacaat
      721 gtgccagagg gactggcagt gggagtggga ttcggagcaa tcgagaagac cgcatccgcc
      781 acatttgagt ctgccagaaa cctggccatc ggcatcggca tccagaattt ccctgagggc
      841 ctggccgtgt ctctgccact gaggggagca ggcttcagca cctggagggc cttttggtat
      901 ggccagctgt ccggaatggt ggagcctctg gcaggcgtgt tcggagcctt tgccgtggtg
      961 ctggcagagc caatcctgcc ttacgcactg gccttcgccg caggagccat ggtgtatgtg
     1021 gtcatggacg atatcatccc agaggcccag atctccggca acggcaagct ggcctcttgg
     1081 gccagcatcc tgggctttgt ggtcatgatg tctctggatg tgggcctggg ctaagaccca
     1141 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1201 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1261 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1321 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1381 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1441 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1501 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1561 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1621 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1681 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1741 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1801 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1861 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1921 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1981 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2041 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2101 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2161 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2221 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2281 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2821 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3001 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3061 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3241 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3301 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3361 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3421 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3481 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3541 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
