LOCUS       sequence-369881-        3257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a human siRNA-expressing plasmid targeting CS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3257)
  TITLE     Zeng Yan Lab Plasmids 2022
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSuper-si-CS
FEATURES             Location/Qualifiers
     source          1..3257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(84..103)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     294..315
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     579..601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     593..610
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     594..610
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     620..639
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        620..638
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     743..759
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     744..760
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(775..989)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(835..854)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(1009..1025)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1009..1025)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1062..1082)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1062..1080)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1101..1117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1101..1117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1114..1136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1125..1141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1149..1155,1156..1173,1174..1179))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1194..1215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1332..1349)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1503..2091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1583..1602)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2262..3053,3054..3122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2885..2904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3123..3227)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3254..3257,1..453)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
       61 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      121 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      181 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      241 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      301 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      361 gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa aaatttaacg
      421 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
      481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      601 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta cccgggatct
      661 agaaaaaaac tcgaggcgac agagtttgct ctggaaacat ccgtgaagcc acagaggatg
      721 tttccagagc agactctgtc gctcgaggtc gacggtatcg ataagcttag atctgtggtc
      781 tcatacagaa cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc
      841 agaacacata gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc
      901 ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc
      961 cttggagcgg gttgatgacg tcagcgttcg aattcctgca gcccggggga tccactagtt
     1021 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     1081 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1141 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1201 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1261 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1321 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1381 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1441 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1501 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1561 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1621 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1681 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1741 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1801 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1861 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1921 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1981 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2041 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2101 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2161 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2221 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2281 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2341 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2401 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2461 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2521 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2581 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2641 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2701 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2761 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2821 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2881 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2941 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3001 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3061 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3121 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3181 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3241 aaagtgccac ctgacgc
//
