LOCUS       sequence-369883-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon optimized SeAvs1 with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: pCDF-SeAvs1
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(455..1537)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1499..1518
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1538..1615)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1661..1680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1661..1679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1680..1704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7484..7501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7547..7594
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7765..8556)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(8557..8648)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac
       61 ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt
      121 tcgaaggtta agtcagttgg ggaactgctt aaccttcgat caaaccacct ccccaggtgg
      181 ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      241 tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac
      301 ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc cccgcgccca
      361 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
      421 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
      601 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
      661 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
      721 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
      781 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
      841 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
      901 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
      961 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1021 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1081 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1141 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     1201 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     1261 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     1321 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     1381 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     1441 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     1501 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     1561 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     1621 tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact cactataggg
     1681 gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt taataaggag
     1741 atataccatg gcgattaaga acagcgcgat catttatgcg ggctacgatt atcagaccct
     1801 gcaaggtgtt cgtctgctgg cggactggct gaacaccccg accaagtaca accgtatcgc
     1861 gttcgaagcg gatgcgaaac aggttgacgc gccgcaaggc attgacgaca tcgtgtgcga
     1921 gcgtcaagat ggcaagaccg acttctggca ggtgaaattt accccggata ccgacaagga
     1981 agacaaccag ctgagctggg agtggctgct gaagcgtagc ggccacagca ttcgtgcgcg
     2041 tagcatcctg caaaaaattg cggatgcggt ggacaaggtt ccggcggaac gtcgtggtga
     2101 catcaccctg ctgaccaaca aaattccgaa ccgtgagatc gcgacctgcc tgcgtaacaa
     2161 caagattgac tggaaccagg ttccgatcgc gaaacagcaa agcatcattc tgcagctggg
     2221 cacccaagaa cgtgcgaagc agttctttga tattctgcaa atctgccaca gcgaccagag
     2281 ctatacccgt ctgaacagca ttgttccgga actgctgcgt aagcacacca acgaggaagg
     2341 tgtgtaccgt ctgatcgagc gtgcgaaacg ttgggcgatt cagcgtaaca gcccgagcga
     2401 tggtggctgg atctgcctgg aacacatccg tgcggtgatt agcaccaacc gtccggagcc
     2461 gatcccgcaa accttcgttc tgccggacaa ctatattgtg ccggatgcgg acttccacga
     2521 taaatttatc gacagcctgt ttaacccgac caaccgtctg gtggttctga ccggtgcgcc
     2581 gggcaagggc aaaagcacct acattagcca catctgccag attctgcaaa cccgtgaatt
     2641 cccgtacatc cgtcaccact attttctggg tctggacgat cgtaccaccg atcgtctgag
     2701 cccgcgtatt gttgcggagg acctgatgtg ccaagtgaaa gcgttctgca gccagatcga
     2761 aatgaagaac tatcacgcgg agcacctgca caaagttctg gcggagtgcg gccagatcta
     2821 caaggaagag ggtaaacgtt tctttatcat tatcgatggc ctggaccacg tgtggcgtga
     2881 taacggcaag gacaaaagcc cgctggatga actgttctgc caactgctgc cgctgccgga
     2941 caacgttacc ctgctggttg gtacccagcc ggttgacgat gagctgctgc cgagccgtct
     3001 gctgcaaaac agcccgcgtg aggaatggct gcacctgccg aacatgagcg gtgacgcgat
     3061 tcgtaagtat ctgagcggcc aggttgaaag cggtcgtatc gtgttcaact ttcaccagag
     3121 ccaatacgag gaagtgctga gccagtgcgc ggagctgctg accaccaaaa cccaaggtta
     3181 tccgctgcac gttatctaca gctgcgaaaa gctgcacgtg gagggtaaag gcctgagcca
     3241 ctgggagatt gaaaacctgc cgcgttgcga aggtggcaac atcaccaact actataacga
     3301 actgtggaag atcctgaact atgagcagcg tgacattctg cacctgtgct gcgcgttccc
     3361 gtttctgtgg ccggcgacca gcttcagcga gatctttagc gaacgtaccg agaccattcc
     3421 gaacgtgaaa gcggttatcc acctgctgta cgaaagcatt gcgggtctgc gtccgttcca
     3481 cgagagcctg attgttttta cccgtagcac caccgagcac gaaaaccgta tcaagctgct
     3541 gctgccggcg ctgattagct ggctggagaa aagcgcgccg aagccgatca aaaactgctg
     3601 gtactggagc tgcctggcgt acaacggcga tccgtatccg ctgcgtaacg gtctgacccg
     3661 tgactggatt ctggaacgtc tggcggaggg ctatcgtcaa gatgaattca ttcgtctgct
     3721 gacccaggcg gagaccagcg cgctggcgga aggtcacttt agcgaggcgt accagcaccg
     3781 tagccgtaag acccgtctgc tgaacgcgcg tctgcaaatt tgggacatga gcaccctggg
     3841 tgtttgcagc atgatcaacg cgagcgaagc gctgctgaag cagtaccaaa gcacccagaa
     3901 cgtgagcagc ccgaaaattc tggcgaccct ggcgattgcg ctgtggttcc gtaaccactt
     3961 tgacgaggcg aaacgtatca cccgtctggc gctgcaacgt tatagcaacg aaagcagcgt
     4021 gtacaccaac aagaacagcg atgagagccg tgcggacatc cgtctgctga ttaaagcggc
     4081 ggttctgacc gaatgcttcg atgagaagtg gctggcgacc ggcagcgtgc acaaatggag
     4141 cgacagcaac atcaacctgc tgattgaatg cgcggagtac aagagcgaca tcggtctgct
     4201 gttcagcctg cacgacgttt ttaaacagac cgtgatcaag aacaaaattg ttaacgcgat
     4261 cgtgcgtgtt ggcattgtgg aacaaatcga tctggagtat tggccgcact tcagcggtct
     4321 ggacagcgcg ctgctgcgtc tgtacagcca tctgagcacc gcgcacccgt gcagcctgat
     4381 caccgagcag ggcgagagcg aaattggtcg ttatcacgtt cacccggaag tgagctacga
     4441 tgagtggttc tatgacagcc tgttttaccg tctgaacgcg agcggtgatt actgctggct
     4501 gccggttagc accggtgaag gccaggaaga ggtgtctagc cacttcctgc acctgaacga
     4561 ttttagcgac attatcgcgg agagcatggc gctgaacatt cagcaaagct tcagcgactt
     4621 ttgcagcctg atcgcgctgg ttagcgatct gaaggaccac cagatgcaaa tccagcaaaa
     4681 gcgtatgttc tttaagaccg attgggttag catcgcgctg aacctgcacc tgattatgca
     4741 ctgcaagccg gtgaacaccg aggaaatcga cattatcctg aacagcgaac acaccgcgct
     4801 gtaccgtctg cacaaaacca ttctgaactt ccacagccgt gcgtttgaga gcgatgcgat
     4861 cgcgaacttc ctggtttttg aagacggtcg tcagaaggag aaactgcaag aaaccaacga
     4921 gtatctggcg aacaacctgg aactgagcga gatcgcgctg cactacgacc tgaaccagag
     4981 cattttcttt gaacgtgtga agctgtgctg ggattacggt ctgggctatg gtcaccacaa
     5041 agacatcgcg ctgaaccagg ttctgaccgc gatcaaaacc attgcgaccg tggaaccgaa
     5101 gtatgcgctg acccaactgg agcgtgttag cccgctggtg cacaacattt gcgacttcac
     5161 cgatggcgac cacacccagc acagcgttac cgaactgagc gcgctgtatg cgcacctgag
     5221 cccgctgacc ctgagcagca tctacgatag ctatgtgagc gagggtgaat ggtatgatgc
     5281 ggacaacgcg ctgacccaat acctgaagca cgcggacctg agcagcccgt tcgtggaaag
     5341 cctgtgccgt accctgctgg acgatggcca gatcgagatt atccaaaacc gtgcgaaaga
     5401 taacgcgatc ctgaccacct tctggccgga aattctgccg cgtaaaatgg actatagcag
     5461 cagcgcgaag cgtagcctgc gtggtaccga gaagtttgat ccggcgaaaa tcagcccggc
     5521 ggacgttacc aacctgctga acgtgcgtag cagctacgaa aacattccga agtggtacca
     5581 ctattggaag gaccagggca aagtgaccga ggttatcaac gtgctgctgc cgattatcaa
     5641 caacggtctg ccggaatata gcgagttccg ttacatcctg agcgacctgt tcgaagacac
     5701 cctgcgtctg aagggcaaga aatacgcgtt cccgattctg gttcaggagc acatccaacg
     5761 taacggctgg ggtgaatggg gtgagagcga cgatcagacc tatgcgcgtc tggataaagt
     5821 gatccgtctg tacccggaca aaattgacga ttttctgtat aagaccaccc gtctgcacca
     5881 ctacaagacc aaagaggaaa acctggttat tccgggcaac aagctgacct acctgctggt
     5941 gaacgttggt cgtgttgatg aagcgaaaag cctgtgcgag gcgatgatca gcgaggtgga
     6001 agcggagacc cagaacctgc cgctgtgcaa accgcagtgg caatgggaag gcgagctgga
     6061 taacgacatg atcgcggtga agttcattat ccgtcgtctg ttttggccgg ttcaatgcgt
     6121 gaaacacctg gttgcggacc agctgagcca cctgctggtg aacggccaat gcgcggagga
     6181 aattgaaaac ctgctggttg tggagatggg taaccgtcag ctggaaagcg aggtggttga
     6241 catcctgacc gttctgtggc tggcgagcct gaagggttat aaagtgcaaa acaacattag
     6301 cagcttcatc tacgcgcgta gctttctgag cgacgcgctg ctggaggcga tcgttccgaa
     6361 cctgccgaac ctgagccgtt accaggtgct gtataagcac ccggacgatg acggcaacca
     6421 ctacggtttc gaaaaaaccc tgggcaacga gctgccgcac atcttttggg atgaagttaa
     6481 acgtctggag gagaagagcg gtgcgccggc gaagatcctg atgaagaaag agtggaacga
     6541 catttgctat aaccacgttc agcgttggga acgtgtggat tacttctttg gcagcgagcg
     6601 tgacggtttc accatgagct ttagcacccg taacacccgt ttcggtatta gcgcgtatct
     6661 gcgtaccatt aaccgtctga tcaacgaatt tcgtatgccg aaacactacg cggagcacta
     6721 tagcatctgc ctgatgagcg cgaacccgct gttctacagc gtgagcaacc atcgtccggg
     6781 ttggctgccg ctgtggcagt atggtgaaat caccaccaag gagaacgtta aaacctacgt
     6841 ggaggaatgc ctgaacgcgt ttaagaacga acaagagaac agcattctgg gtgcgctgag
     6901 cctgccggtt cgtattgatg agaacaactg gctggacatt accgcggtga tgggcatcca
     6961 gaccgaggaa tacgcgagct tcaaaattca acacgcggat tgcggtcaca gcgttgacag
     7021 cctgctgcag gcgtatcgta acatcaagtt cagctttgcg aaatgggcgg aataccaaaa
     7081 ctgcgtgccg ctgctgggta gcacccgtga gctgctgcgt attgcgcgtt gggacatcat
     7141 gtatgaattt cgtggcctgt tcagctttgg ttgccaggag caagttaccg cgtacccggc
     7201 gaagaaccgt atcaacttcg attatcaggg caaaaccatt ggttacagcg acttttggca
     7261 agcgattccg ctgagcatct acccgaaaga cattcgtagc ccggtggcga cctacaccgc
     7321 gtatgataaa gacctggcgt gcaactggaa gaaccacagc gttctgaaga aaccgaacat
     7381 catgctgtgc gattgcaagg tgctgaaacg tgaaaacagc tacagcccgt tcgagatcag
     7441 cgacattcgt ttccactttg aaagcgagcc gctgggcgga agccatcacc atcatcacca
     7501 cagcagcggc taattaacct aggctgctgc caccgctgag caataactag cataacccct
     7561 tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca
     7621 cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc
     7681 tatttaacga ccctgccctg aaccgacgac cgggtcatcg tggccggatc ttgcggcccc
     7741 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
     7801 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     7861 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     7921 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     7981 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     8041 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     8101 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     8161 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     8221 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     8281 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     8341 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     8401 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     8461 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     8521 ttgagtcgat acttcggcga tcaccgcttc cctcatactc ttcctttttc aatattattg
     8581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8641 taaacaaata gctagctcac tcggtcgcta cgctccgggc gtgagactgc ggcgggcgct
     8701 gcggacacat acaaagttac ccacagattc cgtggataag caggggacta acatgtgagg
     8761 caaaacagca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc tgccagagtt
     8821 cacataaaca gacgcttttc cggtgcatct gtgggagccg tgaggctcaa ccatgaatct
     8881 gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg cgggtcgctc
     8941 cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc gcctttctcc
     9001 cttacgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg
     9061 tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct
     9121 tatccggtaa ctgttcactt gagtccaacc cggaaaagca cggtaaaacg ccactggcag
     9181 cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt
     9241 gaagtgtgcg ccaa
//
