LOCUS       sequence-36989-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger 
            K
  TITLE     A high-efficiency system for the generation and study of human 
            induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
  PUBMED    18786420
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Sep 11. 3(3):340-5."
COMMENT     Sequence Label: FUdeltaGW-rtTA
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(59..67,68..103,104..106,107..142,143..145,
                     146..181,182..187,188..805))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga attctagttt
       61 acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
      121 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
      181 ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
      241 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa
      301 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
      361 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
      421 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
      481 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
      541 cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
      601 ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
      661 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
      721 tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagcg ccgtttatga
      781 ctttgctctt gtccagtcta gacatggtgg cgaattaatt ctagagtcga cctgcagccc
      841 aagcttcgtc taacaaaaaa
//
