LOCUS       sequence-36991-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     J.C. Anderson Lab Plasmids
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMLLCK-J72034
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(99..116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(178..202)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(352..371)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(436..455)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctacttcgct aggactgagt ggaattcatg agatctatgg cttctatgac tggcggtcag
       61 caaatgggtt aaggatccta actcgctcct caggcttcct cgctcactga ctcgctgcgc
      121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat caattcgacc
      181 cagctttctt gtacaaagtg gttgatcgga tctgctgatc cttcaactca gcaaaagttc
      241 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
      301 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
      361 ttatgagcca tattcaacgg gaaacgtctt gctccaggcc gcgattaaat tccaacatgg
      421 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
      481 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
      541 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
      601 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
      661 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
      721 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
      781 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca at
//
