LOCUS       sequence-369928-        4379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector, pWVO1 origin of replication (from L. lactis sbsp. 
            cremoris Wg2). Replicates in both Gram-Positive and Gram-Negative 
            bacteria. Chloramphenicol- and Erythromycin resistance marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Kok J, van der Vossen JM, Venema G
  TITLE     Construction of plasmid cloning vectors for lactic streptococci 
            which also replicate in Bacillus subtilis and Escherichia coli.
  JOURNAL   Appl Environ Microbiol. 1984 Oct;48(4):726-31. doi: 
            10.1128/aem.48.4.726-731.1984.
  PUBMED    6095756
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/aem.48.4.726-731"; journalName: "Appl Environ Microbiol"; 
            date: "1984-10"; volume: "48"; issue: "4"; pages: "726-31"
COMMENT     Sequence Label: pGK12
FEATURES             Location/Qualifiers
     source          1..4379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1387..1398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgattcacaa aaaataggca cacgaaaaac aagttaaggg atgcagttta tgcatccctt
       61 aacttactta ttaaataatt tatagctatt gaaaagagat aagaattgtt caaagctaat
      121 attgtttaaa tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa
      181 tattttcttg tattctttgt taacccattt cataacgaaa taattatact tttgtttatc
      241 tttgtgtgat attcttgatt tttttctact taatctgata agtgagctat tcactttagg
      301 tttaggatga aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat
      361 taaaagtaat gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac
      421 gattaaataa atctcattag ctatactatc aaaaacaatt ttgcgtatta tatccgtact
      481 tatgttataa ggtatattac catatatttt ataggattgg tttttaggaa atttaaactg
      541 caatatatcc ttgtttaaaa cttggaaatt atcgtgatca acaagtttat tttctgtagt
      601 tttgcataat ttatggtcta tttcaatggc agttacgaaa ttacacctct ttactaattc
      661 aagggtaaaa tggccttttc ctgagccgat ttcaaagata ttatcatgtt catttaatct
      721 tatatttgtc attattttat ctatattatg ttttgaagta ataaagtttt gactgtgttt
      781 tatatttttc tcgttcatta taaccctctt taatttggtt atatgaattt tgcttattaa
      841 cgattcatta taaccactta ttttttgttt ggttgataat gaactgtgct gattacaaaa
      901 atactaaaaa tgcccatatt ttttcctcct tataaaatta gtataattat agcacgagct
      961 ctgataaata tgaacatgat gagtgatcgt taaatttata ctgcaatcgg atgcgattat
     1021 tgaataaaag atatgagaga tttatctaat ttcttttttc ttgtaaaaaa agaaagttct
     1081 taaaggtttt atagttttgg tcgtagagca cacggtttaa cgacttaatt acgaagtaaa
     1141 taagtctagt gtgttagact ttatgaaatc tatatacgtt tatatatatt tattatccga
     1201 ttttttatta aaacgtctca aaatcgtttc tgagacgttt tagcgtttat ttcgtttagt
     1261 tatcggcata atcgttaaaa caggcgttat cgtagcgtaa aagcccttga gcgtagcgtg
     1321 gctttgcagc gaagatgttg tctgttagat tatgaaagcc gatgactgaa tgaaataata
     1381 agcgcagcgc ccttctattt cggttggagg aggctcaagg gagtatgagg gaatgaaatt
     1441 ccctcatggg tttgatttta aaaattgctt gcaattttgc cgagcggtag cgctggaaaa
     1501 tttttgaaaa aaatttggaa tttggaaaaa aatgggggga aaggaagcga attttgcttc
     1561 cgtactacga ccccccatta agtgccgagt gccaattttt gtgccaaaaa cgctctatcc
     1621 caactggctc aagggtttaa ggggtttttc aatcgccaac gaatcgccaa cgttttcgcc
     1681 aacgtttttt ataaatctat atttaagtag ctttattgtt gtttttatga ttacaaagtg
     1741 atacactaac tttataaaat tatttgattg gagtttttta aatggtgatt tcagaatcga
     1801 aaaaaagagt tatgatttct ctgacaaaag agcaagataa aaaattaaca gatatggcga
     1861 aacaaaaagg tttttcaaaa tctgcggttg cggcgttagc tatagaagaa tatgcaagaa
     1921 aggaatcaga acaaaaaaaa taagcgaaag ctcgcgtttt tagaaggata cgagttttcg
     1981 ctacttgttt ttgataaggt aattatatca tggctattaa aaatactaaa gctagaaatt
     2041 ttggattttt attatatcct gactcaattc ctaatgattg gaaagaaaaa ttagagagtt
     2101 tgggcgtatc tatggctgtc agtcctttac acgatatgga cgaaaaaaaa gataaagata
     2161 catggaatag tagtgatgtt atacgaaatg gaaagcacta taaaaaacca cactatcacg
     2221 ttatatatat tgcacgaaat cctgtaacaa tagaaagcgt taggaacaag attaagcgaa
     2281 aattggggaa tagttcagtt gctcatgttg agatacttga ttatatcaaa ggttcatatg
     2341 aatatttgac tcatgaatca aaggacgcta ttgctaagaa taaacatata tacgacaaaa
     2401 aagatatttt gaacattaat gattttgata ttgaccgcta tataacactt gatgaaagcc
     2461 aaaaaagaga attgaagaat ttacttttag atatagtgga tgactataat ttggtaaata
     2521 caaaagattt aatggctttt attcgcctta ggggagcgga gtttggaatt ttaaatacga
     2581 atgatgtaaa agatattgtt tcaacaaact ctagcgcctt tagattatgg tttgagggca
     2641 attatcagtg tggatataga gcaagttatg caaaggttct tgatgctgaa acgggggaaa
     2701 taaaatgaca aacaaagaaa aagagttatt tgctgaaaat gaggaattaa aaaaagaaat
     2761 taaggactta aaagagcgta ttgaaagata cagagaaatg gaagttgaat taagtacaac
     2821 aatagattta ttgagaggag ggattattga ataaataaaa gccccctgac gaaagtcgaa
     2881 gggggttttt attttggttt gatgttgcga ttaatagcaa tacaattgca ataaacaaaa
     2941 tgatcttcct tcaggttatg accatctgtg ccagttcgta atgtctggtc aactttccga
     3001 ctctgagaaa cttctggaat cgctagagaa tttctggaat gggattcagg agtggacaga
     3061 acgacacgga tatatagtgg atgtgtcaaa acgcatacca ttttgaacga tgacctctaa
     3121 taattgttaa tcatgttggt tacgtattta ttaacttctc ctagtattag taattatcat
     3181 ggctgtcatg gcgcattaac ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat
     3241 aagaaaaagg attaattatg agcgaattga attaataata aggtaataga tttacattag
     3301 aaaatgaaag gggattttat gcgtgagaat gttacagtct atcccggcaa tagttaccct
     3361 tattatcaag ataagaaaga aaaggatttt tcgctacgct caaatccttt aaaaaaacac
     3421 aaaagaccac attttttaat gtggtctttt attcttcaac taaagcaccc attagttcaa
     3481 caaacgaaaa ttggataaag tgggatattt ttaaaatata tatttatgtt acagtaatat
     3541 tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc tcctaaattc
     3601 actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg atttagacaa
     3661 ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac
     3721 cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta
     3781 ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg
     3841 ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt atacaatttt
     3901 tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga
     3961 gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc
     4021 caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac
     4081 tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg
     4141 aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc
     4201 tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag
     4261 gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt
     4321 tctaatgtca ctaacctgcc ccgttagttg aagaaggttt ttatattaca gctccagat
//
