LOCUS       sequence-369929-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SREBF1 - Sterol gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE29
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2048..2079
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2106..2115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2112..3761
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3765..3884
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3975..4563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4028..4048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4446..4457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4578..4894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4745..4880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4945..4947,4948..4950,4951..5664)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4990..5011)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5251..5270)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5598..5619
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5736..5969
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6041..6162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6078..6097)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6132..6151
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6202..6337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6357..6376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6358..6376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6386..6403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6386..6402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6395..6417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6544..6999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6631..6650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6841..6862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7025..7129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7130..7198,7199..7990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7348..7367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8161..8749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8650..8669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8903..8920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9037..9058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9073..9078,9079..9096,9097..9103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9111..9127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9116..9138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9135..9151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9135..9151
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9170..9190
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9172..9190
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcatcac cccacatcac cccacatcac cccacatcac
     1981 cccacatcac cccacatcac cccacgatcc gagaacagat ctggcctcgg cggccaagct
     2041 tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc ggtactgttg
     2101 gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
     2161 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
     2221 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
     2281 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
     2341 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
     2401 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
     2461 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
     2521 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
     2581 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
     2641 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     2701 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     2761 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     2821 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     2881 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     2941 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     3001 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     3061 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     3121 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     3181 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     3241 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     3301 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     3361 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     3421 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     3481 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     3541 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     3601 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     3661 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     3721 agattctcat taaggccaag aagggcggca agatcgccgt gaattctcac ggcttccctc
     3781 ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag ctgcgcccag gagagcggca
     3841 tggatagaca ccctgctgct tgcgccagcg ccaggatcaa cgtctaaggc cgcgactcta
     3901 gaacgcgtca ctgcggtatc agtgtttttc atgcgactac agacatcagc gcgtaccggt
     3961 tagtaatgat cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4561 cgcctggcga tggtaccggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4801 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4861 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4921 tttcggatct gatcagcacc tagcatggtg agcaagggcg aggagctgtt caccggggtg
     4981 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5041 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5101 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
     5161 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5221 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5281 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5341 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat
     5401 atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc
     5461 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5521 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5581 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5641 ggcatggacg agctgtacaa gtaaggtacc tttaagacca atgacttaca aggcagctgt
     5701 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5761 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5821 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5881 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5941 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6001 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6061 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6121 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6181 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6301 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     6361 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6541 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6601 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6661 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6721 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6781 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6841 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6901 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6961 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8821 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8881 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8941 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9001 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9061 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9121 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9181 tcactaaagg gaacaaaagc tggagctgca agctt
//
