LOCUS       sequence-369931-        2778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage ZL19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_ZL19
FEATURES             Location/Qualifiers
     source          1..2778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      729..756
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        775..866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(867..935,936..1727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1085..1104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1898..2486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2387..2406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2640..2657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2738..2757)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agcgttctat ccagtgacat tcttgactca atcaaggatt acaccaactg tgaacgactt
       61 gctacgcaat kactgaaacg taagcagcaa gcggtatgga aagccaaagg tctggctgaa
      121 ctgtgtgatg atagcgaagg atttggtgca gcaagattac gcctggccca ggttgacaac
      181 aatagcggtg ttggtcaggc gattggcatt gacgcagaat ctgaagagta cagcgttcta
      241 aactctgata ttggcggcat cgacgcgttt ctggataaga agttcgatag aatcgttgct
      301 ctgagcggca ttcatgaaat catcctgaag aacaagaacg ttggaggggt atcatcaagc
      361 cagaatacgg cactggagac attccacaag ctggttgacc gcaagcgcaa tgctgagttg
      421 ctccctattc ttgagttctt gatcccgttc atcgcgaacg cagaagagtg gtcagttgag
      481 tttaatccgt tggctcagga atcaagcaag gataaggccg agattcttga gaagaacgca
      541 aacagcattg cggcactaat tgcggcaggc gctatggata tcgacgaggc tcgtgataca
      601 ctacgaacta tagccccaga ggttaagatc aatgatggtt cagtagagac aaaggtgaca
      661 attagtgaaa ccagtaacga cccgttggaa gtacccacag acaattaatg ccaggcatca
      721 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
      781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      841 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      901 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      961 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1021 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1081 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1141 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1201 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1261 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1321 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1381 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1441 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1501 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1561 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1621 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1681 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1741 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1801 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1861 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1921 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1981 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2041 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2101 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2161 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2221 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2281 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2341 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2401 atctttatag ycctgtcggg tttcgccrcc tctgacttga gcgtcgattt ttgtgatgct
     2461 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2521 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2581 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2641 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2701 tgtgcggtat ttcacvccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     2761 agttaagcca gtatacac
//
