LOCUS       sequence-369933-        5273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from phage CBA120.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_CBA120
FEATURES             Location/Qualifiers
     source          1..5273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..31
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    46..186
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(112..131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     272..294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(450..1328)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1355..1639
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1562..1581
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3376..3403
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3422..3513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3514..3582,3583..4374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3732..3751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4545..5133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5034..5053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
       61 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      121 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
      181 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      241 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      301 ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat
      361 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
      421 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
      481 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
      541 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
      601 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
      661 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
      721 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
      781 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
      841 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
      901 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
      961 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag
     1021 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
     1081 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
     1141 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
     1201 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
     1261 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
     1321 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
     1381 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg
     1441 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
     1501 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
     1561 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     1621 ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa ccatggctgg
     1681 atatgggatg ggttttttcg gattgtttgg tggcggcggt ctggtcaatg cgaaagtgga
     1741 tactgataaa ctcgctcaaa aacaggatga actgttgttg accaaagcca cggttgtggc
     1801 tctagacgac gcccaagatg gttctatcat cttacagggt ggcgcaaata cctataacta
     1861 cgtgggcgtg gagtccgaac tgctgagcgt gaaaaccgtt gttgaggagt accaatctat
     1921 ggcgcaacaa ccggaaatcc gcaaggctgt ggacatcatt gtgaacgatg tcgtgacgtg
     1981 cgaagaagat gagacaccgg ttacggtcaa cctggataaa gttgaaggta tcagcgacac
     2041 cgttaaagag tcgattactg agtgctttaa agaagttatg cacctgatgg acttcgacaa
     2101 caccgcatac cagaaaatcc gcaaatggta tgttgacggc cgtcaggcgt accatgttat
     2161 tgtcgatccg accaataaaa aaggcggtat taaaaagcta gtaatgctgg actcccgctg
     2221 catccgtccg gtgtatattg tcgagaaggc catgcgcgag ggcgggattg aagcgattga
     2281 aagcgttact ttgaagtatt actacaaccc gaattacaat cgtaaccagt ttaccggtca
     2341 gagcggcacc tcccaaaact tccagccgtc gcaacaagag ttggtttttg atgacgagag
     2401 catcgtgtac atcgatagcg gtgaagagcc gttagcgaac ggtatcgtgc cgggtttgct
     2461 gaatccggca atccgcccac tgaacaactt ggtgaccacc gaagatgcga ccgtgatcta
     2521 tgcaattacg cgtgcccctg agaaacgtgc gttttatctg gatgtcggaa ccctgggcaa
     2581 gaagagcgct gaagagtaca tgaccatgat gatgggcaag tttaagaacc gtaacgcgta
     2641 cgacagaaca accggtaaga tcactggtaa tgcgcacctg atgggtattg cagaggacta
     2701 ttggctgccg cgtcgtgagg gccagaacgc gacggaaatc gccaccgttg gtggaggcaa
     2761 ccaattaggc gaaatggacc acgttaacta cttccgcgag aagctttatg atgcgctgat
     2821 gattccgaaa tcccgtctgc aagaggaagg ttccattaac attggtggtt ctaatctggc
     2881 tgaaatcacc caggaggaac ttcgtttcag caaattttgt gcaggtttgc ggagacgtta
     2941 tagccatttc ttcatggaat tcctgcgccg tcagctgatt ctgaagggcg ttacggacga
     3001 aaaggactgg aatgaaaaga tcaagccgtt catcaaattc gagttcacct ccgactcata
     3061 tatccgcgaa cagcaagaaa atgccatcct caacgaccgt ctggcgagcc tgaacaccgt
     3121 agagccattt gttggctcga tttttagcat cgactacgtt atgcgtaatg tactgcgtat
     3181 gagcgatgag gaggtgaaag agcagcagac caagatagcg gaggagaaga agaagggtct
     3241 gtacccgaag gtgcaggcgg atgaaaccgg caactacagc ggttcagatg ttagcccgct
     3301 gaaattcaag ccggaaacca ttccgttctc gggcagcacc gacgactcta tttaatgcca
     3361 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     3421 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3481 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3541 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3601 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3661 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3721 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     3781 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3841 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3901 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3961 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4021 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4081 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4141 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4201 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4261 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4321 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4381 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4441 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4501 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4561 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4621 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4681 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4741 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4801 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4861 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4921 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4981 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5041 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5101 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5161 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5221 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccg
//
