LOCUS       sequence-369935-        5192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Portal protein from Desulfurococcales archaeon ex4484_217_2 
            prophage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Portal_prophage_Desulfurococcales_archaeon
FEATURES             Location/Qualifiers
     source          1..5192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(256..1134)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1161..1445
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1368..1387
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3101..3128
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3147..3238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3239..3307,3308..4099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3457..3476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4270..4858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4759..4778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5012..5029
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5044..5184
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5110..5129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
       61 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      121 acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg
      181 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
      241 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
      301 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
      361 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
      421 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
      481 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
      541 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
      601 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
      661 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
      721 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
      781 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
      841 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
      901 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
      961 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     1021 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     1081 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     1141 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
     1201 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat
     1261 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
     1321 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
     1381 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
     1441 tccatacccg ttttttgggc taacaggagg aattaaccat gaacatcgaa ctgcagagcc
     1501 cgaaagtgcg taaactgatt gaggaattcg gttacgcgaa aacctggtac gaacaatatg
     1561 aaaaggagcg tagcgagttt gaccaggatt gggaagacgc gagcgagctg accctgccgt
     1621 acctgtatcc gccgggtatc agcagcggca gcgatattca agagcagctg ccgaccccgt
     1681 ataacagcat tggtccggcg agcgttaacg cgctggcgag caaactgctg ctggcgctgc
     1741 tgccgccgac cggcgtgttc tttcgtctgc tgccggacga tgaggaagtt accgacctga
     1801 gcgaggaaga gctgaagcag ctggacaaag aactgagcaa gctggagcaa gatgtggttc
     1861 agtacatcaa cgaaaaagcg ctgcgtattc cggtgttcga ggcgctgaag tacctgatcg
     1921 tgaccggtaa cgttggcatg tataaagtgc cgaacggtag cttcaaggtt tttgcgccgt
     1981 accaatatgt ggttcagcgt gactatgttg gtaacatcct gaccgcggcg atcaaagaac
     2041 gtattggctt taacgcgctg ccgcaagacg tgcaggaaaa gattgaagag gcggttgatg
     2101 agaacaccaa cgaagagcaa agcgaaaaca aagaggtgga tatctacacc ttcattctgc
     2161 gtaccaacaa caacagcttt accgtttggc aggaaatcaa caaatatgtg ctggaggaca
     2221 ccgttaagac ctacaccaag gataaactgc cgtatatctt cctgcgttgg accaccatta
     2281 acagcgagga ctacggtcgt ggccacgtgc agcaatatct gggtgatctg cgtagcctgg
     2341 aaggcctgac ccagagcatc gttgagggtg cgggcattag cgcgctgacc ctgtttggtg
     2401 tgcgtccggg tagcaccctg aaagttgaag acctgaacaa cgcgagcaac ggcgagtttg
     2461 tgctgggcga tctggaacgt gagatcagca ccctgcaaat tcagaaggcg ggtgactacc
     2521 aagttgcgat gaccctgatc cagcaactgg agcagcgtct ggcgcgtgcg tttctgatgc
     2581 tgggtggcca aattcgtgat agcgaacgta ccaccgcgac cgaggtgcgc caagttgcgg
     2641 cggaactgga ggcgaccctg ggtggcgtgt acagcgttct ggcggcggaa ttccaaaaac
     2701 cgctgatcac cctggtgctg caggagatga tgccggacgt tctgaagttt accaccccga
     2761 ccattaccac cggcatcacc gcgattagcc gtgaacgtga ttaccagaac ctgaacgtga
     2821 tggttcaaac catcgcgcag ctgggtccgg aagtgatgag ccaatatctg aacattagcg
     2881 gcttcctgag ccagctggcg accagcctgg gtatggaccc ggcgggcgtg gttcgtaccg
     2941 atgaagagat ccaggcgatt cagcaacagc aacagcaaat gatgatgcag caacaggcga
     3001 tgcaacaggc gctgcaaacc ggtggcaaag tggttgaaga gggtgcgcgt aacagcaaca
     3061 agggtaccca gaacggctaa tgccaggcat caaattaagc agaaggccat cctgacggat
     3121 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     3181 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3241 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3301 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3361 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3421 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3481 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3541 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3601 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3661 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3721 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3781 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4141 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4381 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4921 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4981 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5041 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     5101 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     5161 ctacgtgact gggtcatggc tgcgccccga ca
//
