LOCUS       sequence-369936-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIF1a - Hypoxia gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE9
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2064..2095
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2122..2131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2128..3777
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3781..3900
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3992..4580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4045..4065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4463..4474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4595..4911
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4762..4897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4962..4964,4965..4967,4968..5681)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5007..5028)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5268..5287)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5615..5636
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5753..5986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6058..6179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6095..6114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6149..6168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6219..6354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6374..6393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6375..6393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6403..6420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6403..6419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6412..6434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6561..7016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6648..6667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6858..6879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7042..7146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7147..7215,7216..8007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7365..7384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8178..8766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8667..8686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8920..8937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9054..9075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9090..9095,9096..9113,9114..9120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9128..9144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9133..9155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9152..9168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9152..9168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9187..9207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9189..9207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgtgac tacgtgctgc ctaggtgact acgtgctgcc
     1981 taggtgacta cgtgctgcct aggtgactac gtgctgccta ggatccgaga acagatctgg
     2041 cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg
     2101 caatccggta ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca
     2161 gcgccattct acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag
     2221 cgctacgccc tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt
     2281 acctacgccg agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg
     2341 ctgaatacaa accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc
     2401 gtgttgggtg ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag
     2461 cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa
     2521 gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc
     2581 atggatagca agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat
     2641 ttgccacccg gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc
     2701 atcgccctga tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg
     2761 caccgcaccg cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc
     2821 atccccgaca ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc
     2881 acgctgggct acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag
     2941 ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta
     3001 tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag
     3061 atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc
     3121 cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc
     3181 acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct
     3241 aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc
     3301 gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc
     3361 atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac
     3421 ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca
     3481 gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc
     3541 ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa
     3601 accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag
     3661 ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac
     3721 gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgaat
     3781 tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc
     3841 gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc
     3901 taaggccgcg actctagaac gcgtcactgc ggctagaacc ctgcgggttt ggactacaga
     3961 catcagcgcg taccggttag taatgatcga caatcaacct ctggattaca aaatttgtga
     4021 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4081 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4141 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4201 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4261 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4321 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4381 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4441 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4501 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4561 cctttgggcc gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca
     4621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4741 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4921 ggagcttgta tatccatttt cggatctgat cagcacctag catggtgagc aagggcgagg
     4981 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5041 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5101 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct
     5161 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5221 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5281 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5341 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5401 acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca
     5461 agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5521 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5581 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5641 ccgccgggat cactctcggc atggacgagc tgtacaagta aggtaccttt aagaccaatg
     5701 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5761 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5821 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5881 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5941 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6001 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6181 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6241 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6301 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     6361 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6421 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6481 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6541 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6901 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     7021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7381 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7681 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8881 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8941 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9061 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9121 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9181 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc tt
//
