LOCUS       sequence-37002-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon 
            AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams 
            A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR
  TITLE     Alternative splicing regulates mouse embryonic stem cell 
            pluripotency and differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 
            24.
  PUBMED    20498046
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-06-8"; volume: "107(23)"; pages: 
            "10514-9. Epub 2010 May 24"
COMMENT     Sequence Label: pSicoR-Ef1a-mCh-GFPi
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(304..337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        537..748
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gccgantcaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
       61 tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg
      121 ccatctctag gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc
      181 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      241 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      301 tctataactt cgtatagcat acattatacg aagttataca tgtcacaaaa ggaaactcac
      361 cctaactgta aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg
      421 tttacagcca caacgtctat atttcaagag aatatagacg ttgtggctgt ttttttctcg
      481 agtactagga tccattaggc ggccgcggat ctgcgatcgc tccggtgccc gtcagtgggc
      541 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      601 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      661 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      721 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
      781 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
      841 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
      901 gagaccgggc ctttgtcc
//
