LOCUS       sequence-370025-        6012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for Camk2g expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Li H, Ou J, Li Y, Xu N, Li Q, Wu P, Peng C, Tang YC, Chang HC
  TITLE     ISX-9 potentiates CaMKIIdelta-mediated BMAL1 activation to enhance 
            circadian amplitude.
  JOURNAL   Commun Biol. 2022 Jul 28;5(1):750. doi: 10.1038/s42003-022-03725-x.
  PUBMED    35902736
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-07-28"; volume: "5(1):750. doi"; pages: " 
            10.1038/s42003-022-03725-x"
COMMENT     Sequence Label: pGl3-Camk2g
FEATURES             Location/Qualifiers
     source          1..6012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1276..1285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1282..2934
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1348..1366)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2975..3096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2986..3005)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3040..3059
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3344..3361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3515..4103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3595..3614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4274..5065,5066..5134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4897..4916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5135..5239)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5266..5721
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5353..5372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5563..5584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5852..5900
                     /note="synthetic polyadenylation signal"
     misc_feature    5914..6005
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5954..5973
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt ccagacatcc gagagctagg tagtatgatc caagagttct
       61 caatactgtg tgctagcact catacttgca aatacatcat tacaaaatgt gataaagtgt
      121 cataaataaa agtactggat gcccacaaga aaacgagcat gttttcatct tttgctttta
      181 gccactagtg cacaaagcct tttcaagaca ttcttacttt tatcttttat tactgaccta
      241 ggagagaaca tttatttcta agactggaat gcaggaaagc atcacacggg cccgcaggac
      301 agctcagtga gtaaaaagat gcgtgagcta agcctgacta cccaagttcc atcccccaat
      361 gtagtgaaaa gagaaccaac tccccaagtt gtgtgacctc cacacgtgcg ctgtggcaac
      421 catagggcca caggcataca cacacgaaac aaataaatgg aaagaaagaa agaaagaaag
      481 aaagaaagaa agaaagaaag aaaggaagga aggaagaaag aaagaaagaa aagcaagcaa
      541 acatcttgcc ccatggcctg gtttcgctga taaaaccgcg gctgaaggtt ttctggtttt
      601 gttttttctt aatgatgccc ttgattcttg cactaatcta gagtgtcggc accctggggg
      661 aaggctgcgg gcagctggct ctgcagtctg ggggcatctc tcgagtgtac agtctcacat
      721 acgctctttg accttggcat tttccggctt ggttcaggct gcagtcatgg gacccacgtg
      781 tggggcctct ccccagacct cggtggtcca gacccaacac cactcaagaa ccactttcac
      841 acattggttc ctgagctctt gcagggtccg ccctggagga gcccgcagga ggtgtcttcc
      901 agttcggtcg gtcccagtgg gtggccggga gtggggaccc cgcgtccttg gccctcgggt
      961 gcagggggcc cagttcgggc ggctcctggg gcggggcggg gccgacggtg ggaggcccct
     1021 ccccgggggc ggggcggggg ccagcggggc ttgggggcgg ggccggcggg cgggcgcggg
     1081 ggtgtccctc cctccctcgc tctctccggt aaagtctcgc ggtgctgccg ggctcagccc
     1141 cgtctcctcc tcttgctccc tcggccgggc ggcggtgact gtgcaccgac gtcggcgcgg
     1201 gctgcaccgc cgcgtccgcc cgccagtata gatctgcgat ctaagtaagc ttggcattcc
     1261 ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg cccggcgcca
     1321 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     1381 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac
     1441 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     1501 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     1561 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     1621 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg
     1681 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat
     1741 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     1801 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca
     1861 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga
     1921 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     1981 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     2041 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     2101 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc
     2161 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     2221 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg
     2281 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc
     2341 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     2401 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga
     2461 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     2521 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     2581 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa
     2641 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc
     2701 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     2761 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     2821 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     2881 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag
     2941 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3001 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3061 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3121 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3181 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
     3241 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     3301 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     3361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     5281 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     5341 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     5401 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     5461 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     5521 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     5581 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5641 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     5701 aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc
     5761 gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata
     5821 ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct
     5881 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
     5941 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
     6001 tctctatcga ta
//
