LOCUS       sequence-37003-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon 
            AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams 
            A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR
  TITLE     Alternative splicing regulates mouse embryonic stem cell 
            pluripotency and differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 
            24.
  PUBMED    20498046
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-06-8"; volume: "107(23)"; pages: 
            "10514-9. Epub 2010 May 24"
COMMENT     Sequence Label: pSicoR-Ef1a-mCh-Puro-GFPi
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(305..338)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        538..749
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aacngnntca actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
       61 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
      121 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
      181 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
      241 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
      301 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca
      361 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt
      421 gtttacagcc acaacgtcta tatttcaaga gaatatagac gttgtggctg tttttttctc
      481 gagtactagg atccattagg cggccgcgga tctgcgatcg ctccggtgcc cgtcagtggg
      541 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
      601 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      661 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      721 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
      781 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
      841 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
//
