LOCUS       sequence-370044-        9209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAT3 - Stat3 homodimer gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE5
FEATURES             Location/Qualifiers
     source          1..9209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2042..2073
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2100..2109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2106..3755
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3759..3878
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3969..4557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4022..4042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4440..4451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4572..4888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4739..4874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4939..4941,4942..4944,4945..5658)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4984..5005)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5245..5264)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5592..5613
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5730..5963
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6035..6156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6072..6091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6126..6145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6196..6331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6351..6370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6352..6370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6380..6397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6380..6396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6389..6411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6538..6993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6625..6644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6835..6856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7019..7123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7124..7192,7193..7984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7342..7361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8155..8743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8644..8663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8897..8914
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9031..9052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9067..9072,9073..9090,9091..9097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9105..9121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9110..9132
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9129..9145
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9129..9145
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9164..9184
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9166..9184
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctgctt cccgaattcc cgaattcccg aattcccgaa
     1981 ttcccgaatt cccgaacgtg atccgagaac agatctggcc tcggcggcca agcttagaca
     2041 ctagagggta tataatggaa gctcgacttc cagcttggca atccggtact gttggtaaag
     2101 ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag
     2161 acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca
     2221 ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga
     2281 tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg
     2341 tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg
     2401 gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg
     2461 gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg
     2521 tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc
     2581 agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt
     2641 acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta
     2701 gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat
     2761 tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca
     2821 gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg
     2881 gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag
     2941 actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca
     3001 ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc
     3061 tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg
     3121 gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc
     3181 ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg
     3241 gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga
     3301 gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc
     3361 acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga
     3421 agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc
     3481 tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg
     3541 agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg
     3601 tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg
     3661 tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc
     3721 tcattaaggc caagaagggc ggcaagatcg ccgtgaattc tcacggcttc cctcccgagg
     3781 tggaggagca ggccgccggc accctgccca tgagctgcgc ccaggagagc ggcatggata
     3841 gacaccctgc tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac tctagaacgc
     3901 gtcactgcgg ctggaattaa tatcaacacg atacagacat cagcgcgtac cggttagtaa
     3961 tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4021 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4081 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4141 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4201 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4261 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4321 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     4381 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4441 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4501 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     4561 gcgatggtac cggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4621 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4681 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4801 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     4861 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     4921 atctgatcag cacctagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4981 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     5041 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5101 cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcgcccgc
     5161 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5221 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5281 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5341 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     5401 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     5461 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5521 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5581 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5641 gacgagctgt acaagtaagg tacctttaag accaatgact tacaaggcag ctgtagatct
     5701 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5761 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5941 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6001 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     6061 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6121 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     6181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6301 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     6361 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6421 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6481 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     6541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6961 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     7021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8281 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8941 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9001 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9061 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9121 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     9181 aagggaacaa aagctggagc tgcaagctt
//
