LOCUS       sequence-370051-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE57
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2063..2094
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2121..2130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2127..3776
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3780..3899
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3991..4579
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4044..4064)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4462..4473)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4594..4910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4761..4896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4961..4963,4964..4966,4967..5680)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5006..5027)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5267..5286)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5614..5635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5752..5985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6057..6178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6094..6113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6148..6167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6218..6353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6373..6392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6374..6392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6402..6419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6402..6418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6411..6433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6560..7015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6647..6666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6857..6878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7041..7145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7146..7214,7215..8006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7364..7383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8177..8765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8666..8685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8919..8936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9053..9074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9089..9094,9095..9112,9113..9119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9127..9143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9132..9154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9151..9167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9151..9167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9186..9206
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9188..9206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatctagtt tcactttccc tagtttcact ttccctagtt
     1981 tcactttccc tagtttcact ttccctagtt tcactttccc gatccgagaa cagatctggc
     2041 ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt ccagcttggc
     2101 aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag
     2161 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
     2221 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
     2281 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
     2341 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
     2401 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
     2461 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
     2521 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     2581 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     2641 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     2701 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     2761 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     2821 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     2881 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     2941 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     3001 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     3061 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     3121 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     3181 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     3241 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     3301 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     3361 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     3421 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     3481 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     3541 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     3601 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     3661 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     3721 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt
     3781 ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc atgagctgcg
     3841 cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg atcaacgtct
     3901 aaggccgcga ctctagaacg cgtcactgcg gctctccggt ggccgaccat gactacagac
     3961 atcagcgcgt accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa
     4021 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4081 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4141 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4201 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4261 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4321 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4381 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4441 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4501 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4561 ctttgggccg cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4921 gagcttgtat atccattttc ggatctgatc agcacctagc atggtgagca agggcgagga
     4981 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5041 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5101 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
     5161 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5221 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5281 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5341 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5401 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5461 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5521 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5581 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5641 cgccgggatc actctcggca tggacgagct gtacaagtaa ggtaccttta agaccaatga
     5701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6001 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6181 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6301 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6361 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6421 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6481 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6541 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6601 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6661 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6721 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6781 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6841 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6901 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6961 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9001 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9061 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9181 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
